Eukaryotic initiation factor eIF6 modulates the expression of Kermit 2/XGIPC in IGF- regulated eye development

N. De Marco, M. Tussellino, R. Carotenuto, R. Ronca, S. Rizzolio, S. Biffo, C. Campanella

Research output: Contribution to journalArticle

Abstract

The eukaryotic initiation translation factor eIF6 is a highly conserved, essential protein implicated in translation. eIF6 is regulated in vivo by extracellular signals, such as IGF signaling (for a review see Miluzio et al., 2009). In Xenopus, eif6 over-expression causes a delay in eye development (De Marco et al., 2011). In this study we showed that eif6 co-immunoprecipitates with the insulin-like growth factor receptor (igfr) and may function downstream of igf in eye formation. The relationship between eif6 and gipc2, a protein partner of a variety of molecules including membrane proteins, was investigated. gipc2 is required for maintaining igf-induced akt activation on eye development (Wu et al., 2006). Significantly eif6 and gipc2 have opposite effects in eye development. While eif6 is required for eye formation below threshold levels, gipc2 knockdown impairs eye development (De Marco et al., 2011; Wu et al., 2006). In this study, it was shown that in eif6 over-expressors, the delay in eye morphogenesis is reversed by gipc2 injection, while the injection of eif6 down-regulates gipc2 expression. Real-time-PCR indicates that eif6 regulates gipc2 expression in a dose-dependent manner. In contrast, gipc2 knockdown has no significant effect on eif6 mRNA levels. These results suggest that eif6 regulation of gipc2 enables correct morphogenesis of Xenopus eye and stimulate questions on the molecular network implicated in this process.

Original languageEnglish
Pages (from-to)148-154
Number of pages7
JournalDevelopmental Biology
Volume427
Issue number1
DOIs
Publication statusPublished - Jul 1 2017

Fingerprint

Eukaryotic Initiation Factors
Xenopus
Morphogenesis
Somatomedin Receptors
Injections
Real-Time Polymerase Chain Reaction
Membrane Proteins
Proteins
Down-Regulation
Messenger RNA

Keywords

  • Eukaryotic initiation factor eif6
  • Eye field
  • igf
  • Kermit 2/Xgipc

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

Eukaryotic initiation factor eIF6 modulates the expression of Kermit 2/XGIPC in IGF- regulated eye development. / De Marco, N.; Tussellino, M.; Carotenuto, R.; Ronca, R.; Rizzolio, S.; Biffo, S.; Campanella, C.

In: Developmental Biology, Vol. 427, No. 1, 01.07.2017, p. 148-154.

Research output: Contribution to journalArticle

De Marco, N. ; Tussellino, M. ; Carotenuto, R. ; Ronca, R. ; Rizzolio, S. ; Biffo, S. ; Campanella, C. / Eukaryotic initiation factor eIF6 modulates the expression of Kermit 2/XGIPC in IGF- regulated eye development. In: Developmental Biology. 2017 ; Vol. 427, No. 1. pp. 148-154.
@article{f9567807eab54950babdd4a17dfcf67b,
title = "Eukaryotic initiation factor eIF6 modulates the expression of Kermit 2/XGIPC in IGF- regulated eye development",
abstract = "The eukaryotic initiation translation factor eIF6 is a highly conserved, essential protein implicated in translation. eIF6 is regulated in vivo by extracellular signals, such as IGF signaling (for a review see Miluzio et al., 2009). In Xenopus, eif6 over-expression causes a delay in eye development (De Marco et al., 2011). In this study we showed that eif6 co-immunoprecipitates with the insulin-like growth factor receptor (igfr) and may function downstream of igf in eye formation. The relationship between eif6 and gipc2, a protein partner of a variety of molecules including membrane proteins, was investigated. gipc2 is required for maintaining igf-induced akt activation on eye development (Wu et al., 2006). Significantly eif6 and gipc2 have opposite effects in eye development. While eif6 is required for eye formation below threshold levels, gipc2 knockdown impairs eye development (De Marco et al., 2011; Wu et al., 2006). In this study, it was shown that in eif6 over-expressors, the delay in eye morphogenesis is reversed by gipc2 injection, while the injection of eif6 down-regulates gipc2 expression. Real-time-PCR indicates that eif6 regulates gipc2 expression in a dose-dependent manner. In contrast, gipc2 knockdown has no significant effect on eif6 mRNA levels. These results suggest that eif6 regulation of gipc2 enables correct morphogenesis of Xenopus eye and stimulate questions on the molecular network implicated in this process.",
keywords = "Eukaryotic initiation factor eif6, Eye field, igf, Kermit 2/Xgipc",
author = "{De Marco}, N. and M. Tussellino and R. Carotenuto and R. Ronca and S. Rizzolio and S. Biffo and C. Campanella",
year = "2017",
month = "7",
day = "1",
doi = "10.1016/j.ydbio.2017.04.017",
language = "English",
volume = "427",
pages = "148--154",
journal = "Developmental Biology",
issn = "0012-1606",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Eukaryotic initiation factor eIF6 modulates the expression of Kermit 2/XGIPC in IGF- regulated eye development

AU - De Marco, N.

AU - Tussellino, M.

AU - Carotenuto, R.

AU - Ronca, R.

AU - Rizzolio, S.

AU - Biffo, S.

AU - Campanella, C.

PY - 2017/7/1

Y1 - 2017/7/1

N2 - The eukaryotic initiation translation factor eIF6 is a highly conserved, essential protein implicated in translation. eIF6 is regulated in vivo by extracellular signals, such as IGF signaling (for a review see Miluzio et al., 2009). In Xenopus, eif6 over-expression causes a delay in eye development (De Marco et al., 2011). In this study we showed that eif6 co-immunoprecipitates with the insulin-like growth factor receptor (igfr) and may function downstream of igf in eye formation. The relationship between eif6 and gipc2, a protein partner of a variety of molecules including membrane proteins, was investigated. gipc2 is required for maintaining igf-induced akt activation on eye development (Wu et al., 2006). Significantly eif6 and gipc2 have opposite effects in eye development. While eif6 is required for eye formation below threshold levels, gipc2 knockdown impairs eye development (De Marco et al., 2011; Wu et al., 2006). In this study, it was shown that in eif6 over-expressors, the delay in eye morphogenesis is reversed by gipc2 injection, while the injection of eif6 down-regulates gipc2 expression. Real-time-PCR indicates that eif6 regulates gipc2 expression in a dose-dependent manner. In contrast, gipc2 knockdown has no significant effect on eif6 mRNA levels. These results suggest that eif6 regulation of gipc2 enables correct morphogenesis of Xenopus eye and stimulate questions on the molecular network implicated in this process.

AB - The eukaryotic initiation translation factor eIF6 is a highly conserved, essential protein implicated in translation. eIF6 is regulated in vivo by extracellular signals, such as IGF signaling (for a review see Miluzio et al., 2009). In Xenopus, eif6 over-expression causes a delay in eye development (De Marco et al., 2011). In this study we showed that eif6 co-immunoprecipitates with the insulin-like growth factor receptor (igfr) and may function downstream of igf in eye formation. The relationship between eif6 and gipc2, a protein partner of a variety of molecules including membrane proteins, was investigated. gipc2 is required for maintaining igf-induced akt activation on eye development (Wu et al., 2006). Significantly eif6 and gipc2 have opposite effects in eye development. While eif6 is required for eye formation below threshold levels, gipc2 knockdown impairs eye development (De Marco et al., 2011; Wu et al., 2006). In this study, it was shown that in eif6 over-expressors, the delay in eye morphogenesis is reversed by gipc2 injection, while the injection of eif6 down-regulates gipc2 expression. Real-time-PCR indicates that eif6 regulates gipc2 expression in a dose-dependent manner. In contrast, gipc2 knockdown has no significant effect on eif6 mRNA levels. These results suggest that eif6 regulation of gipc2 enables correct morphogenesis of Xenopus eye and stimulate questions on the molecular network implicated in this process.

KW - Eukaryotic initiation factor eif6

KW - Eye field

KW - igf

KW - Kermit 2/Xgipc

UR - http://www.scopus.com/inward/record.url?scp=85019162858&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85019162858&partnerID=8YFLogxK

U2 - 10.1016/j.ydbio.2017.04.017

DO - 10.1016/j.ydbio.2017.04.017

M3 - Article

C2 - 28472630

AN - SCOPUS:85019162858

VL - 427

SP - 148

EP - 154

JO - Developmental Biology

JF - Developmental Biology

SN - 0012-1606

IS - 1

ER -