European emergence of ciprofloxacin-resistant Escherichia coli clonal groups O25: H4-ST 131 and O15:K52:H1 causing community-acquired uncomplicated cystitis

Simone Cagnacci, Laura Gualco, Eugenio Debbia, Gian Carlo Schito, Anna Marchese

Research output: Contribution to journalArticle

Abstract

A total of 148 E. coli strains displaying reduced susceptibility to ciprofloxacin (MIC ≥ 2 μg/ml) and causing uncomplicated urinary tract infections in eight European countries during 2003 to 2006 were studied. Their phylogenetic groups, biochemical profiles, and antibiotic susceptibilities were determined. Determination of the O:H serotype, pulsed-field gel electrophoresis (PFGE), randomly amplified polymorphic DNA (RAPD) PCR, and multilocus sequence typing provided additional discrimination. The majority (82.4%) of the microorganisms (122/148) carried resistance to two or more additional drugs, with the pattern ciprofloxacin-trimethoprim-sufamethoxazole-tetracycline- ampicillin being the most represented (73 strains out of 148; 49.3%). Extended-spectrum beta-lactamase production was detected in 12/148 strains (8.1%), with CTX-M-15 being the most-common enzyme. Six strains out of the whole collection studied (4.0%) contained a qnrB-like gene. Overall, 55 different PFGE or RAPD PCR profiles could be distinguished, indicating a substantial heterogeneity. However, about one-third (51/148) of the strains belonged to two clonal groups: O15:K52:H1 (phylogenetic group B2, lactose-nonfermenting variant, ciprofloxacin MIC of 16 μg/ml) and O25:H4 sequence type 131 (ST-131) (phylogenetic group D, ciprofloxacin MIC of ≥32 μg/ml). With the exception of Poland, strains of these two groups were isolated in samples from all participating countries but more frequently in samples from Spain and Italy. In some representative strains of the two main clonal groups, alterations in GyrA and ParC were the basic mechanism of fluoroquinolone resistance. In some members of the O25:H4 ST-131 group, displaying a ciprofloxacin MIC of >32 μg/ml, additional OmpF loss or pump efflux overexpression was found. In the Mediterranean area, strains belonging to these two clonal groups played a major role in determining the high rate of fluoroquinolone-resistant E. coli strains observed in the community.

Original languageEnglish
Pages (from-to)2605-2612
Number of pages8
JournalJournal of Clinical Microbiology
Volume46
Issue number8
DOIs
Publication statusPublished - Aug 2008

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Cystitis
Ciprofloxacin
Escherichia coli
Pulsed Field Gel Electrophoresis
Fluoroquinolones
Multilocus Sequence Typing
Polymerase Chain Reaction
Trimethoprim
DNA
Poland
Ampicillin
Lactose
beta-Lactamases
Tetracycline
Urinary Tract Infections
Spain
Italy
Anti-Bacterial Agents
Enzymes
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

European emergence of ciprofloxacin-resistant Escherichia coli clonal groups O25 : H4-ST 131 and O15:K52:H1 causing community-acquired uncomplicated cystitis. / Cagnacci, Simone; Gualco, Laura; Debbia, Eugenio; Schito, Gian Carlo; Marchese, Anna.

In: Journal of Clinical Microbiology, Vol. 46, No. 8, 08.2008, p. 2605-2612.

Research output: Contribution to journalArticle

Cagnacci, Simone ; Gualco, Laura ; Debbia, Eugenio ; Schito, Gian Carlo ; Marchese, Anna. / European emergence of ciprofloxacin-resistant Escherichia coli clonal groups O25 : H4-ST 131 and O15:K52:H1 causing community-acquired uncomplicated cystitis. In: Journal of Clinical Microbiology. 2008 ; Vol. 46, No. 8. pp. 2605-2612.
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abstract = "A total of 148 E. coli strains displaying reduced susceptibility to ciprofloxacin (MIC ≥ 2 μg/ml) and causing uncomplicated urinary tract infections in eight European countries during 2003 to 2006 were studied. Their phylogenetic groups, biochemical profiles, and antibiotic susceptibilities were determined. Determination of the O:H serotype, pulsed-field gel electrophoresis (PFGE), randomly amplified polymorphic DNA (RAPD) PCR, and multilocus sequence typing provided additional discrimination. The majority (82.4{\%}) of the microorganisms (122/148) carried resistance to two or more additional drugs, with the pattern ciprofloxacin-trimethoprim-sufamethoxazole-tetracycline- ampicillin being the most represented (73 strains out of 148; 49.3{\%}). Extended-spectrum beta-lactamase production was detected in 12/148 strains (8.1{\%}), with CTX-M-15 being the most-common enzyme. Six strains out of the whole collection studied (4.0{\%}) contained a qnrB-like gene. Overall, 55 different PFGE or RAPD PCR profiles could be distinguished, indicating a substantial heterogeneity. However, about one-third (51/148) of the strains belonged to two clonal groups: O15:K52:H1 (phylogenetic group B2, lactose-nonfermenting variant, ciprofloxacin MIC of 16 μg/ml) and O25:H4 sequence type 131 (ST-131) (phylogenetic group D, ciprofloxacin MIC of ≥32 μg/ml). With the exception of Poland, strains of these two groups were isolated in samples from all participating countries but more frequently in samples from Spain and Italy. In some representative strains of the two main clonal groups, alterations in GyrA and ParC were the basic mechanism of fluoroquinolone resistance. In some members of the O25:H4 ST-131 group, displaying a ciprofloxacin MIC of >32 μg/ml, additional OmpF loss or pump efflux overexpression was found. In the Mediterranean area, strains belonging to these two clonal groups played a major role in determining the high rate of fluoroquinolone-resistant E. coli strains observed in the community.",
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