European LeukemiaNet study on the reproducibility of bone marrow features in masked polycythemia vera and differentiation from essential thrombocythemia

Hans Michael Kvasnicka, Attilio Orazi, Juergen Thiele, Giovanni Barosi, Carlos E. Bueso-Ramos, Alessandro M. Vannucchi, Robert P. Hasserjian, Jean Jacques Kiladjian, Umberto Gianelli, Richard Silver, Tariq I. Mughal, Tiziano Barbui

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The purpose of the study was to assess consensus and interobserver agreement among an international panel of six hematopathologists regarding characterization and reproducibility of bone marrow (BM) histologic features used to diagnose early stage myeloproliferative neoplasms, in particular differentiation of so-called masked/prodromal polycythemia vera (mPV) from JAK2-mutated essential thrombocythemia (ET). The six members of the hematopathology panel evaluated 98 BM specimens independently and in a blinded fashion without knowledge of clinical data. The specimens included 48 cases of mPV according to the originally published hemoglobin threshold values for this entity (male: 16.0–18.4 g/dL, female: 15.0–16.4 g/dL), 31 cases with overt PV according to the updated 2016 WHO criteria, and 19 control cases. The latter group included cases of JAK2-mutated ET, primary myelofibrosis, myelodysplastic syndrome, and various reactive conditions. Inter-rater agreement between the panelists was very high (overall agreement 92.6%, kappa 0.812), particularly with respect to separating mPV from ET. Virtually all cases of mPV were correctly classified as PV according to their BM morphology. In conclusion, a central blinded review of histology slides by six hematopathologists demonstrated that highly reproducible specific histological pattern characterize PV and confirmed the notion that there are no significant differences between mPV and overt PV in relation to BM morphology.

Original languageEnglish
Pages (from-to)1062-1067
Number of pages6
JournalAmerican Journal of Hematology
Volume92
Issue number10
DOIs
Publication statusPublished - Oct 1 2017

Fingerprint

Essential Thrombocythemia
Polycythemia Vera
Bone Marrow
Primary Myelofibrosis
Myelodysplastic Syndromes
Histology
Hemoglobins
Neoplasms

ASJC Scopus subject areas

  • Hematology

Cite this

European LeukemiaNet study on the reproducibility of bone marrow features in masked polycythemia vera and differentiation from essential thrombocythemia. / Kvasnicka, Hans Michael; Orazi, Attilio; Thiele, Juergen; Barosi, Giovanni; Bueso-Ramos, Carlos E.; Vannucchi, Alessandro M.; Hasserjian, Robert P.; Kiladjian, Jean Jacques; Gianelli, Umberto; Silver, Richard; Mughal, Tariq I.; Barbui, Tiziano.

In: American Journal of Hematology, Vol. 92, No. 10, 01.10.2017, p. 1062-1067.

Research output: Contribution to journalArticle

Kvasnicka, HM, Orazi, A, Thiele, J, Barosi, G, Bueso-Ramos, CE, Vannucchi, AM, Hasserjian, RP, Kiladjian, JJ, Gianelli, U, Silver, R, Mughal, TI & Barbui, T 2017, 'European LeukemiaNet study on the reproducibility of bone marrow features in masked polycythemia vera and differentiation from essential thrombocythemia', American Journal of Hematology, vol. 92, no. 10, pp. 1062-1067. https://doi.org/10.1002/ajh.24837
Kvasnicka, Hans Michael ; Orazi, Attilio ; Thiele, Juergen ; Barosi, Giovanni ; Bueso-Ramos, Carlos E. ; Vannucchi, Alessandro M. ; Hasserjian, Robert P. ; Kiladjian, Jean Jacques ; Gianelli, Umberto ; Silver, Richard ; Mughal, Tariq I. ; Barbui, Tiziano. / European LeukemiaNet study on the reproducibility of bone marrow features in masked polycythemia vera and differentiation from essential thrombocythemia. In: American Journal of Hematology. 2017 ; Vol. 92, No. 10. pp. 1062-1067.
@article{d8c9737251e54ff5a83f68ce3f0fc99c,
title = "European LeukemiaNet study on the reproducibility of bone marrow features in masked polycythemia vera and differentiation from essential thrombocythemia",
abstract = "The purpose of the study was to assess consensus and interobserver agreement among an international panel of six hematopathologists regarding characterization and reproducibility of bone marrow (BM) histologic features used to diagnose early stage myeloproliferative neoplasms, in particular differentiation of so-called masked/prodromal polycythemia vera (mPV) from JAK2-mutated essential thrombocythemia (ET). The six members of the hematopathology panel evaluated 98 BM specimens independently and in a blinded fashion without knowledge of clinical data. The specimens included 48 cases of mPV according to the originally published hemoglobin threshold values for this entity (male: 16.0–18.4 g/dL, female: 15.0–16.4 g/dL), 31 cases with overt PV according to the updated 2016 WHO criteria, and 19 control cases. The latter group included cases of JAK2-mutated ET, primary myelofibrosis, myelodysplastic syndrome, and various reactive conditions. Inter-rater agreement between the panelists was very high (overall agreement 92.6{\%}, kappa 0.812), particularly with respect to separating mPV from ET. Virtually all cases of mPV were correctly classified as PV according to their BM morphology. In conclusion, a central blinded review of histology slides by six hematopathologists demonstrated that highly reproducible specific histological pattern characterize PV and confirmed the notion that there are no significant differences between mPV and overt PV in relation to BM morphology.",
author = "Kvasnicka, {Hans Michael} and Attilio Orazi and Juergen Thiele and Giovanni Barosi and Bueso-Ramos, {Carlos E.} and Vannucchi, {Alessandro M.} and Hasserjian, {Robert P.} and Kiladjian, {Jean Jacques} and Umberto Gianelli and Richard Silver and Mughal, {Tariq I.} and Tiziano Barbui",
year = "2017",
month = "10",
day = "1",
doi = "10.1002/ajh.24837",
language = "English",
volume = "92",
pages = "1062--1067",
journal = "American Journal of Hematology",
issn = "0361-8609",
publisher = "Wiley-Liss Inc.",
number = "10",

}

TY - JOUR

T1 - European LeukemiaNet study on the reproducibility of bone marrow features in masked polycythemia vera and differentiation from essential thrombocythemia

AU - Kvasnicka, Hans Michael

AU - Orazi, Attilio

AU - Thiele, Juergen

AU - Barosi, Giovanni

AU - Bueso-Ramos, Carlos E.

AU - Vannucchi, Alessandro M.

AU - Hasserjian, Robert P.

AU - Kiladjian, Jean Jacques

AU - Gianelli, Umberto

AU - Silver, Richard

AU - Mughal, Tariq I.

AU - Barbui, Tiziano

PY - 2017/10/1

Y1 - 2017/10/1

N2 - The purpose of the study was to assess consensus and interobserver agreement among an international panel of six hematopathologists regarding characterization and reproducibility of bone marrow (BM) histologic features used to diagnose early stage myeloproliferative neoplasms, in particular differentiation of so-called masked/prodromal polycythemia vera (mPV) from JAK2-mutated essential thrombocythemia (ET). The six members of the hematopathology panel evaluated 98 BM specimens independently and in a blinded fashion without knowledge of clinical data. The specimens included 48 cases of mPV according to the originally published hemoglobin threshold values for this entity (male: 16.0–18.4 g/dL, female: 15.0–16.4 g/dL), 31 cases with overt PV according to the updated 2016 WHO criteria, and 19 control cases. The latter group included cases of JAK2-mutated ET, primary myelofibrosis, myelodysplastic syndrome, and various reactive conditions. Inter-rater agreement between the panelists was very high (overall agreement 92.6%, kappa 0.812), particularly with respect to separating mPV from ET. Virtually all cases of mPV were correctly classified as PV according to their BM morphology. In conclusion, a central blinded review of histology slides by six hematopathologists demonstrated that highly reproducible specific histological pattern characterize PV and confirmed the notion that there are no significant differences between mPV and overt PV in relation to BM morphology.

AB - The purpose of the study was to assess consensus and interobserver agreement among an international panel of six hematopathologists regarding characterization and reproducibility of bone marrow (BM) histologic features used to diagnose early stage myeloproliferative neoplasms, in particular differentiation of so-called masked/prodromal polycythemia vera (mPV) from JAK2-mutated essential thrombocythemia (ET). The six members of the hematopathology panel evaluated 98 BM specimens independently and in a blinded fashion without knowledge of clinical data. The specimens included 48 cases of mPV according to the originally published hemoglobin threshold values for this entity (male: 16.0–18.4 g/dL, female: 15.0–16.4 g/dL), 31 cases with overt PV according to the updated 2016 WHO criteria, and 19 control cases. The latter group included cases of JAK2-mutated ET, primary myelofibrosis, myelodysplastic syndrome, and various reactive conditions. Inter-rater agreement between the panelists was very high (overall agreement 92.6%, kappa 0.812), particularly with respect to separating mPV from ET. Virtually all cases of mPV were correctly classified as PV according to their BM morphology. In conclusion, a central blinded review of histology slides by six hematopathologists demonstrated that highly reproducible specific histological pattern characterize PV and confirmed the notion that there are no significant differences between mPV and overt PV in relation to BM morphology.

UR - http://www.scopus.com/inward/record.url?scp=85026454556&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85026454556&partnerID=8YFLogxK

U2 - 10.1002/ajh.24837

DO - 10.1002/ajh.24837

M3 - Article

C2 - 28685840

AN - SCOPUS:85026454556

VL - 92

SP - 1062

EP - 1067

JO - American Journal of Hematology

JF - American Journal of Hematology

SN - 0361-8609

IS - 10

ER -