Evaluating B-cells: From bone marrow precursors to antibody-producing cells

M. Manuela Rosado, Marco Scarsella, Simona Cascioli, Ezio Giorda, Rita Carsetti

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Lymphocyte characterization is primarily based on the differential expression of surface markers. In this context, flow-cytometry analysis (FACS) is an exceptional technique that not only allows the identification of B-cell subsets, but can also be used to evaluate cell function, activation, and division. Here, we will combine the use of FACS analysis and ELISA techniques to identify murine bone marrow and peripheral B-cell subsets. The main function of B cells, derived through a multistage differentiation process from precursor cells, is to produce antibodies. This task is performed by terminally differentiated B cells called antibody-secreting cells (ASC) present at mucosal sites, in the bone marrow and in the spleen. The number and specificity of ASC can be measured by Enzyme-linked immunosorbent spot (ELISPOT) assay, a variation of the enzyme-linked immunosorbent assay (ELISA) used to quantify serum immunoglobulins.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages45-57
Number of pages13
Volume1032
ISBN (Print)9781627034951
DOIs
Publication statusPublished - 2013

Publication series

NameMethods in Molecular Biology
Volume1032
ISSN (Print)10643745

Keywords

  • Antibodies
  • Flow-cytometry
  • Mouse B cell subsets

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Medicine(all)

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