TY - CHAP
T1 - Evaluating B-cells
T2 - From bone marrow precursors to antibody-producing cells
AU - Rosado, M. Manuela
AU - Scarsella, Marco
AU - Cascioli, Simona
AU - Giorda, Ezio
AU - Carsetti, Rita
PY - 2013
Y1 - 2013
N2 - Lymphocyte characterization is primarily based on the differential expression of surface markers. In this context, flow-cytometry analysis (FACS) is an exceptional technique that not only allows the identification of B-cell subsets, but can also be used to evaluate cell function, activation, and division. Here, we will combine the use of FACS analysis and ELISA techniques to identify murine bone marrow and peripheral B-cell subsets. The main function of B cells, derived through a multistage differentiation process from precursor cells, is to produce antibodies. This task is performed by terminally differentiated B cells called antibody-secreting cells (ASC) present at mucosal sites, in the bone marrow and in the spleen. The number and specificity of ASC can be measured by Enzyme-linked immunosorbent spot (ELISPOT) assay, a variation of the enzyme-linked immunosorbent assay (ELISA) used to quantify serum immunoglobulins.
AB - Lymphocyte characterization is primarily based on the differential expression of surface markers. In this context, flow-cytometry analysis (FACS) is an exceptional technique that not only allows the identification of B-cell subsets, but can also be used to evaluate cell function, activation, and division. Here, we will combine the use of FACS analysis and ELISA techniques to identify murine bone marrow and peripheral B-cell subsets. The main function of B cells, derived through a multistage differentiation process from precursor cells, is to produce antibodies. This task is performed by terminally differentiated B cells called antibody-secreting cells (ASC) present at mucosal sites, in the bone marrow and in the spleen. The number and specificity of ASC can be measured by Enzyme-linked immunosorbent spot (ELISPOT) assay, a variation of the enzyme-linked immunosorbent assay (ELISA) used to quantify serum immunoglobulins.
KW - Antibodies
KW - Flow-cytometry
KW - Mouse B cell subsets
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U2 - 10.1007/978-1-62703-496-8_4
DO - 10.1007/978-1-62703-496-8_4
M3 - Chapter
C2 - 23943443
AN - SCOPUS:84922832640
SN - 9781627034951
VL - 1032
T3 - Methods in Molecular Biology
SP - 45
EP - 57
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -