Evaluating the effects of an organic extract from the mediterranean sponge Geodia cydonium on human breast cancer cell lines

Susan Costantini, Eliana Guerriero, Roberta Teta, Francesca Capone, Alessia Caso, Angela Sorice, Giovanna Romano, Adrianna Ianora, Nadia Ruocco, Alfredo Budillon, Valeria Costantino, Maria Costantini

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Marine sponges are an excellent source of bioactive secondary metabolites for pharmacological applications. In the present study, we evaluated the chemistry, cytotoxicity and metabolomics of an organic extract from the Mediterranean marine sponge Geodia cydonium, collected in coastal waters of the Gulf of Naples. We identified an active fraction able to block proliferation of breast cancer cell lines MCF-7, MDA-MB231, and MDA-MB468 and to induce cellular apoptosis, whereas it was inactive on normal breast cells (MCF-10A). Metabolomic studies showed that this active fraction was able to interfere with amino acid metabolism, as well as to modulate glycolysis and glycosphingolipid metabolic pathways. In addition, the evaluation of the cytokinome profile on the polar fractions of three treated breast cancer cell lines (compared to untreated cells) demonstrated that this fraction induced a slight anti-inflammatory effect. Finally, the chemical entities present in this fraction were analyzed by liquid chromatography high resolution mass spectrometry combined with molecular networking.

Original languageEnglish
Article number2112
JournalInternational Journal of Molecular Sciences
Volume18
Issue number10
DOIs
Publication statusPublished - Oct 1 2017

Fingerprint

Geodia
Rosaceae
Metabolomics
Porifera
cultured cells
breast
cancer
Cells
Breast Neoplasms
Cell Line
Glycosphingolipids
Liquid chromatography
multiple docking adapters
Glycolysis
Cell death
Cytotoxicity
Metabolites
Metabolic Networks and Pathways
Metabolism
Liquid Chromatography

Keywords

  • Breast cancer
  • Cytokines
  • Cytotoxicity
  • Metabolomics
  • Sponges

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

Evaluating the effects of an organic extract from the mediterranean sponge Geodia cydonium on human breast cancer cell lines. / Costantini, Susan; Guerriero, Eliana; Teta, Roberta; Capone, Francesca; Caso, Alessia; Sorice, Angela; Romano, Giovanna; Ianora, Adrianna; Ruocco, Nadia; Budillon, Alfredo; Costantino, Valeria; Costantini, Maria.

In: International Journal of Molecular Sciences, Vol. 18, No. 10, 2112, 01.10.2017.

Research output: Contribution to journalArticle

Costantini, S, Guerriero, E, Teta, R, Capone, F, Caso, A, Sorice, A, Romano, G, Ianora, A, Ruocco, N, Budillon, A, Costantino, V & Costantini, M 2017, 'Evaluating the effects of an organic extract from the mediterranean sponge Geodia cydonium on human breast cancer cell lines', International Journal of Molecular Sciences, vol. 18, no. 10, 2112. https://doi.org/10.3390/ijms18102112
Costantini, Susan ; Guerriero, Eliana ; Teta, Roberta ; Capone, Francesca ; Caso, Alessia ; Sorice, Angela ; Romano, Giovanna ; Ianora, Adrianna ; Ruocco, Nadia ; Budillon, Alfredo ; Costantino, Valeria ; Costantini, Maria. / Evaluating the effects of an organic extract from the mediterranean sponge Geodia cydonium on human breast cancer cell lines. In: International Journal of Molecular Sciences. 2017 ; Vol. 18, No. 10.
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