TY - JOUR
T1 - Evaluating the effects of an organic extract from the mediterranean sponge Geodia cydonium on human breast cancer cell lines
AU - Costantini, Susan
AU - Guerriero, Eliana
AU - Teta, Roberta
AU - Capone, Francesca
AU - Caso, Alessia
AU - Sorice, Angela
AU - Romano, Giovanna
AU - Ianora, Adrianna
AU - Ruocco, Nadia
AU - Budillon, Alfredo
AU - Costantino, Valeria
AU - Costantini, Maria
PY - 2017/10/1
Y1 - 2017/10/1
N2 - Marine sponges are an excellent source of bioactive secondary metabolites for pharmacological applications. In the present study, we evaluated the chemistry, cytotoxicity and metabolomics of an organic extract from the Mediterranean marine sponge Geodia cydonium, collected in coastal waters of the Gulf of Naples. We identified an active fraction able to block proliferation of breast cancer cell lines MCF-7, MDA-MB231, and MDA-MB468 and to induce cellular apoptosis, whereas it was inactive on normal breast cells (MCF-10A). Metabolomic studies showed that this active fraction was able to interfere with amino acid metabolism, as well as to modulate glycolysis and glycosphingolipid metabolic pathways. In addition, the evaluation of the cytokinome profile on the polar fractions of three treated breast cancer cell lines (compared to untreated cells) demonstrated that this fraction induced a slight anti-inflammatory effect. Finally, the chemical entities present in this fraction were analyzed by liquid chromatography high resolution mass spectrometry combined with molecular networking.
AB - Marine sponges are an excellent source of bioactive secondary metabolites for pharmacological applications. In the present study, we evaluated the chemistry, cytotoxicity and metabolomics of an organic extract from the Mediterranean marine sponge Geodia cydonium, collected in coastal waters of the Gulf of Naples. We identified an active fraction able to block proliferation of breast cancer cell lines MCF-7, MDA-MB231, and MDA-MB468 and to induce cellular apoptosis, whereas it was inactive on normal breast cells (MCF-10A). Metabolomic studies showed that this active fraction was able to interfere with amino acid metabolism, as well as to modulate glycolysis and glycosphingolipid metabolic pathways. In addition, the evaluation of the cytokinome profile on the polar fractions of three treated breast cancer cell lines (compared to untreated cells) demonstrated that this fraction induced a slight anti-inflammatory effect. Finally, the chemical entities present in this fraction were analyzed by liquid chromatography high resolution mass spectrometry combined with molecular networking.
KW - Breast cancer
KW - Cytokines
KW - Cytotoxicity
KW - Metabolomics
KW - Sponges
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UR - http://www.scopus.com/inward/citedby.url?scp=85031088882&partnerID=8YFLogxK
U2 - 10.3390/ijms18102112
DO - 10.3390/ijms18102112
M3 - Article
AN - SCOPUS:85031088882
VL - 18
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
SN - 1661-6596
IS - 10
M1 - 2112
ER -