Evaluation by double-blind placebo-controlled oral challenge of the clinical relevance of IgE antibodies against plant glycans

A. Mari, P. Ooievaar-De Heer, E. Scala, M. Giani, L. Pirrotta, L. Zuidmeer, D. Bethell, R. Van Ree

Research output: Contribution to journalArticlepeer-review

Abstract

Background: The clinical relevance of immunoglobulin E (IgE) to plant glycans is a longstanding debate. We sought to evaluate their clinical reactivity using the human glycoprotein lactoferrin expressed in rice. Methods: Allergic patients with IgE antibodies against plant glycans were analyzed for the presence of IgE against rice-produced lactoferrin. The potency of IgE to induce mediator release was assessed by basophil histamine release and skin prick tests (SPTs). Clinical relevance was evaluated by double-blind placebo-controlled oral challenge (DBPCOC). Results: Twenty-four of 29 sera (82.7%) with IgE antibodies against plant glycans demonstrated IgE binding to transgenic lactoferrin. In three of five cases transgenic lactoferrin induced histamine release. Compared to a control major grass pollen allergen lactoferrin concentrations needed for biological activity of IgE were 5-6 orders of magnitude higher. Skin prick test and DBPCOC were negative in five patients with potential clinical reactivity that volunteered to undergo these in vivo challenges. Conclusions: Poor or no biological activity and lack of clinical relevance of IgE-binding plant glycans (five out of five) was demonstrated using human lactoferrin expressed in rice as a model.

Original languageEnglish
Pages (from-to)891-896
Number of pages6
JournalAllergy: European Journal of Allergy and Clinical Immunology
Volume63
Issue number7
DOIs
Publication statusPublished - Jul 2008

Keywords

  • Basophil histamine release
  • Cross-reactive carbohydrate determinants (CCD)
  • Double-blind placebo-controlled oral challenge
  • IgE
  • Plant glycoproteins
  • Transgenic plants

ASJC Scopus subject areas

  • Immunology

Fingerprint Dive into the research topics of 'Evaluation by double-blind placebo-controlled oral challenge of the clinical relevance of IgE antibodies against plant glycans'. Together they form a unique fingerprint.

Cite this