Evaluation of a commercial DNA probe assay for the identification of Mycobacterium kansasii

E. Tortoli, M. T. Simonetti, C. Lacchini, V. Penati, C. Piersimoni, V. Morbiducci

Research output: Contribution to journalArticlepeer-review

Abstract

A commercially available DNA probe (the Accu-Probe Mycobacterium kansasii culture identification test, Gen-Probe, USA) for the identification of Mycobacterium kansasii was tested on a panel of 143 fully characterized mycobacterial strains. The isolates included 70 Mycobacterium kansasii and 73 mycobacteria other than kansasii. The specificity was 100 % while the sensitivity was 72.8 %. This sensitivity is unusually low in comparison with that of commercial DNA probes for other mycobacteria and confirms a previous study that found genetic heterogeneity within the species Mycobacterium kansasii. Strains that do not hybridize with the AccuProbe are particularly prevalent in Italy and perhaps elsewhere in Europe.

Original languageEnglish
Pages (from-to)264-267
Number of pages4
JournalEuropean Journal of Clinical Microbiology and Infectious Diseases
Volume13
Issue number3
DOIs
Publication statusPublished - Mar 1994

ASJC Scopus subject areas

  • Microbiology (medical)
  • Immunology

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