Evaluation of a commercial serological kit for detection of salivary immunoglobulin G to Helicobacter pylori: A multicentre study

F. Luzza, M. Imeneo, A. Marasco, S. Crotta, E. Ierardi, P. Usai, C. Virgilio, G. Nardone, S. Marchi, G. Sanna, F. Perri, R. M. Zagari, F. Bazzoli

Research output: Contribution to journalArticlepeer-review


Objective: To evaluate the usefulness of a commercial serological enzyme immunoassay (EIA) for detecting salivary immunoglobulin (Ig) G to Helicobacter pylori. Design: Prospective, multicentre study. Setting: The study was conducted at 11 gastroenterology hospital centres throughout Italy. Participants and interventions: Two hundred and thirteen dyspeptic patients underwent gastroscopy with antral biopsies. At each centre, two of the following three tests for H. pylori diagnosis were performed: urease quick test, histology, and 13C-urea breath test. Samples of unstimulated saliva and venous blood were collected from each patient. Salivary and serum H. pylori IgG were determined with the EIA Helori-test IgG (Eurospital, Trieste, Italy). Main outcome measures: Sensitivity, specificity, positive and negative predictive values, and accuracy of the salivary and serum EIAs. Results: By using a receiver operating characteristic curve, salivary EIA yielded 81% (95% confidence interval 73-87%) sensitivity, 73% (62-83%) specificity, 84% (76-90%) positive predictive value, 69% (58-79%) negative predictive value, and 78% (72-84%) accuracy. At the cut-off suggested by the manufacturer, serum EIA had 90% (84-95%) sensitivity, 78% (67-86%) specificity, 88% (81-93%) positive predictive value, 82% (71-90%) negative predictive value, and 86% (81-91%) accuracy. Conclusion: In this large multicentre study, detection of salivary H. pylori IgG with a commercial serological EIA was a fairly accurate diagnostic method. Data confirm that saliva testing does not compare favourably with serology in the assessment of H. pylori status. (C) 2000 Lippincott Williams and Wilkins.

Original languageEnglish
Pages (from-to)1117-1120
Number of pages4
JournalEuropean Journal of Gastroenterology and Hepatology
Issue number10
Publication statusPublished - 2000


  • Diagnosis
  • Helicobacter pylori
  • Saliva testing
  • Salivary IgG
  • Serum IgG

ASJC Scopus subject areas

  • Gastroenterology


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