Evaluation of a new multiplex assay for allergy diagnosis

Buzzulini Francesca, Da Re Mirella, Scala Enrico, Martelli Paola, Conte Mariaelisabetta, Brusca Ignazio, Villalta Danilo

Research output: Contribution to journalArticlepeer-review


Background: Molecular allergy has significantly improved the quality of allergy diagnosis; however, the positioning of singleplex and multiplex assays in the diagnostic algorithm is still a matter of debate. Methods: Aim of the study was to test the analytical performance of the recently commercialized Allergy Explorer-ALEX® in a selected population (105 allergic patients and 15 negative controls), comparing it with the reference ImmunoCAP® method and with skin prick test (SPT). Results: Inter-assay qualitative comparison showed a substantial agreement between ALEX® and SPT (k = 0.64). A substantial agreement between ALEX® and ImmunoCAP® was shown on the detection of IgE to extracts (k = 0.64 for inhalants and k = 0.51 for food allergens), whereas a higher agreement was shown on detection of molecular components (k = 0.92 for inhalants and k = 0.72 for food allergens). Quantitative comparison showed a poor correlation between ALEX® and ImmunoCAP®. Conclusion: The simultaneous detection of both extracts and molecular components with ALEX® assay can potentially overcome some of the major limitations of the multiplex assay currently in use. However, before using ALEX® as routine method, the analytical performance (in particular for extracts) needs to be further investigated on a larger scale.

Original languageEnglish
Pages (from-to)73-78
Number of pages6
JournalClinica Chimica Acta
Publication statusPublished - Jun 2019


  • Allergy explorer-ALEX®
  • Bottom down approach/bottom up approach
  • Molecular allergy
  • Multiplex assays/singleplex assays

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical


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