Evaluation of absorption with Limulus amebocyte lysate to remove contaminating endotoxin from interferon and lymphokine preparations

A. Biondi, S. Landolfo, D. Fumaroa, N. Polentarutti, M. Introna, A. Mantovani

Research output: Contribution to journalArticle

Abstract

α and β human interferon (IFN) preparations and lymphokines (supernatants of PHA-stimulated blood lymphocytes) were deliberately contaminated with endotoxin (20 ng/ml) and subsequently rendered endotoxin-free by absorption with Limulus amebocyte lysate (LAL). Absorption with LAL did not appreciably affect the antiviral activity of IFN and lymphokines in 8 experiments and caused a 30-50% reduction in two. The capacity of these agents to stimulate natural killer cell activity and monocyte cytotoxicity was not consistently modified by absorption on LAL. When the chemotactic activity of lymphokine for monocytes was measured, the maximal number of monocytes induced to migrate and the maximal active lymphokine concentration were not affected by absorption with LAL. LAL-treated lymphokines, however, showed a prozone phenomenon, presumably related to the release of chemotaxis inhibitor(s) from the LAL gel.

Original languageEnglish
Pages (from-to)103-112
Number of pages10
JournalJournal of Immunological Methods
Volume66
Issue number1
DOIs
Publication statusPublished - 1984

Fingerprint

Horseshoe Crabs
Lymphokines
Endotoxins
Interferons
Monocytes
Chemotaxis
Natural Killer Cells
Antiviral Agents
endotoxin binding proteins
Gels
Lymphocytes

Keywords

  • endotoxin
  • interferon
  • Limulus amebocyte lysate
  • lymphokine

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

Cite this

Evaluation of absorption with Limulus amebocyte lysate to remove contaminating endotoxin from interferon and lymphokine preparations. / Biondi, A.; Landolfo, S.; Fumaroa, D.; Polentarutti, N.; Introna, M.; Mantovani, A.

In: Journal of Immunological Methods, Vol. 66, No. 1, 1984, p. 103-112.

Research output: Contribution to journalArticle

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AU - Biondi, A.

AU - Landolfo, S.

AU - Fumaroa, D.

AU - Polentarutti, N.

AU - Introna, M.

AU - Mantovani, A.

PY - 1984

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N2 - α and β human interferon (IFN) preparations and lymphokines (supernatants of PHA-stimulated blood lymphocytes) were deliberately contaminated with endotoxin (20 ng/ml) and subsequently rendered endotoxin-free by absorption with Limulus amebocyte lysate (LAL). Absorption with LAL did not appreciably affect the antiviral activity of IFN and lymphokines in 8 experiments and caused a 30-50% reduction in two. The capacity of these agents to stimulate natural killer cell activity and monocyte cytotoxicity was not consistently modified by absorption on LAL. When the chemotactic activity of lymphokine for monocytes was measured, the maximal number of monocytes induced to migrate and the maximal active lymphokine concentration were not affected by absorption with LAL. LAL-treated lymphokines, however, showed a prozone phenomenon, presumably related to the release of chemotaxis inhibitor(s) from the LAL gel.

AB - α and β human interferon (IFN) preparations and lymphokines (supernatants of PHA-stimulated blood lymphocytes) were deliberately contaminated with endotoxin (20 ng/ml) and subsequently rendered endotoxin-free by absorption with Limulus amebocyte lysate (LAL). Absorption with LAL did not appreciably affect the antiviral activity of IFN and lymphokines in 8 experiments and caused a 30-50% reduction in two. The capacity of these agents to stimulate natural killer cell activity and monocyte cytotoxicity was not consistently modified by absorption on LAL. When the chemotactic activity of lymphokine for monocytes was measured, the maximal number of monocytes induced to migrate and the maximal active lymphokine concentration were not affected by absorption with LAL. LAL-treated lymphokines, however, showed a prozone phenomenon, presumably related to the release of chemotaxis inhibitor(s) from the LAL gel.

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