Abstract
α and β human interferon (IFN) preparations and lymphokines (supernatants of PHA-stimulated blood lymphocytes) were deliberately contaminated with endotoxin (20 ng/ml) and subsequently rendered endotoxin-free by absorption with Limulus amebocyte lysate (LAL). Absorption with LAL did not appreciably affect the antiviral activity of IFN and lymphokines in 8 experiments and caused a 30-50% reduction in two. The capacity of these agents to stimulate natural killer cell activity and monocyte cytotoxicity was not consistently modified by absorption on LAL. When the chemotactic activity of lymphokine for monocytes was measured, the maximal number of monocytes induced to migrate and the maximal active lymphokine concentration were not affected by absorption with LAL. LAL-treated lymphokines, however, showed a prozone phenomenon, presumably related to the release of chemotaxis inhibitor(s) from the LAL gel.
| Original language | English |
|---|---|
| Pages (from-to) | 103-112 |
| Number of pages | 10 |
| Journal | Journal of Immunological Methods |
| Volume | 66 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1984 |
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Keywords
- endotoxin
- interferon
- Limulus amebocyte lysate
- lymphokine
ASJC Scopus subject areas
- Biotechnology
- Immunology
Cite this
Evaluation of absorption with Limulus amebocyte lysate to remove contaminating endotoxin from interferon and lymphokine preparations. / Biondi, A.; Landolfo, S.; Fumaroa, D.; Polentarutti, N.; Introna, M.; Mantovani, A.
In: Journal of Immunological Methods, Vol. 66, No. 1, 1984, p. 103-112.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Evaluation of absorption with Limulus amebocyte lysate to remove contaminating endotoxin from interferon and lymphokine preparations
AU - Biondi, A.
AU - Landolfo, S.
AU - Fumaroa, D.
AU - Polentarutti, N.
AU - Introna, M.
AU - Mantovani, A.
PY - 1984
Y1 - 1984
N2 - α and β human interferon (IFN) preparations and lymphokines (supernatants of PHA-stimulated blood lymphocytes) were deliberately contaminated with endotoxin (20 ng/ml) and subsequently rendered endotoxin-free by absorption with Limulus amebocyte lysate (LAL). Absorption with LAL did not appreciably affect the antiviral activity of IFN and lymphokines in 8 experiments and caused a 30-50% reduction in two. The capacity of these agents to stimulate natural killer cell activity and monocyte cytotoxicity was not consistently modified by absorption on LAL. When the chemotactic activity of lymphokine for monocytes was measured, the maximal number of monocytes induced to migrate and the maximal active lymphokine concentration were not affected by absorption with LAL. LAL-treated lymphokines, however, showed a prozone phenomenon, presumably related to the release of chemotaxis inhibitor(s) from the LAL gel.
AB - α and β human interferon (IFN) preparations and lymphokines (supernatants of PHA-stimulated blood lymphocytes) were deliberately contaminated with endotoxin (20 ng/ml) and subsequently rendered endotoxin-free by absorption with Limulus amebocyte lysate (LAL). Absorption with LAL did not appreciably affect the antiviral activity of IFN and lymphokines in 8 experiments and caused a 30-50% reduction in two. The capacity of these agents to stimulate natural killer cell activity and monocyte cytotoxicity was not consistently modified by absorption on LAL. When the chemotactic activity of lymphokine for monocytes was measured, the maximal number of monocytes induced to migrate and the maximal active lymphokine concentration were not affected by absorption with LAL. LAL-treated lymphokines, however, showed a prozone phenomenon, presumably related to the release of chemotaxis inhibitor(s) from the LAL gel.
KW - endotoxin
KW - interferon
KW - Limulus amebocyte lysate
KW - lymphokine
UR - http://www.scopus.com/inward/record.url?scp=0021325065&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021325065&partnerID=8YFLogxK
U2 - 10.1016/0022-1759(84)90253-9
DO - 10.1016/0022-1759(84)90253-9
M3 - Article
C2 - 6693773
AN - SCOPUS:0021325065
VL - 66
SP - 103
EP - 112
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
SN - 0022-1759
IS - 1
ER -