Valutazione di test supplementari per la conferma di HIV-1 e HIV-2 in donatori di sangue a pazienti

Translated title of the contribution: Evaluation of additional tests for the confirmation of HIV-1 and HIV-2 in blood donors and patients

M. Mazzucco, F. Rizzuto, R. Poma, R. Insolia, L. Salvaneschi

Research output: Contribution to journalArticle

Abstract

This evaluation compares 2 additional tests, HIV Blot 2.2-Genelabs which uses the HIV-1 viral proteins separated by molecular weight and a synthetic peptide (gp36) for HIV-2 and SIA-Ortho Diagnostic Systems which is made entirely from synthetic antigens, recombinant proteins for HIV-1 and (gp36) HIV-2; for the confirmation of an HIV infection evaluating different serological profiles, from seroconversion to the end stage (AIDS), the serological profiles of the Subgroup '0' and HIV-2 included. Results: for specificity, there were no false positive detected by either methods (3 indetermined SIA, 1 indetermined WB.); for sensitivity, it was equal for known seropositives and seroconversion panel (both day 14); the HIV-2 peptide on the HIV Blot 2.2 appeared to he more sensitive in dilution studies than the analogous SIA. For selectivity, SIA detected 1 undetermined and 4 positive out of 5 positive, WB detected 5 positive out of 5; otherwise, both methods detected as positive the gp 36 peptide of all the 7 HIV-2 positive. WB was able to show reactivity to the GAG-POL precursor protein, demonstrating the ability to detect HIV variants. So, this evaluation does not demonstrate distinct advantages of SIA over HIV Blot 2.2, referring to specificity and reduction of indeterminate results; the advantages of HIV Blot 2.2 are related to the use of native viral antigens, representing the entire viral genome which can improve the detection of known and unknown HIV variants and can guarantee a better comparison for false positive results obtained with screening test using synthetic or recombinant antigens.

Translated title of the contributionEvaluation of additional tests for the confirmation of HIV-1 and HIV-2 in blood donors and patients
Original languageItalian
Pages (from-to)16-23
Number of pages8
JournalTrasfusione del Sangue
Volume42
Issue number1
Publication statusPublished - 1997

ASJC Scopus subject areas

  • Hematology
  • Immunology and Allergy

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  • Cite this

    Mazzucco, M., Rizzuto, F., Poma, R., Insolia, R., & Salvaneschi, L. (1997). Valutazione di test supplementari per la conferma di HIV-1 e HIV-2 in donatori di sangue a pazienti. Trasfusione del Sangue, 42(1), 16-23.