Evaluation of an automated spectrophotometric assay for reactive oxygen metabolites in serum

Luigia Iamele, Roberto Fiocchi, Arialdo Vernocchi

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

The in vivo assesment of free radicals concentration is hampered by their instability and extremely short half-life. The Diacron Reactive Oxygen Metabolies (D-ROM) test is a recently introduced method to evaluate the peroxidation of organic compounds. Since the manual performance of the test provides excessive analytical imprecision, the aim of this study was to evaluate the automation, of this test. Within- and between-run imprecision and interference were assessed according to the guidelines proposed by the NCCLS. The resolve oxygen metabolites' (ROM) stability was evaluated in different physical conditions. For within-run and between-run imprecision the coefficients of variation were consistently lower than 5%. The maximum allowable concentration was 28.2 mmol/l, 0.068 mmol/l and 171 mmol/l for triglycerides, haemoglobin and bilirubin, respectively. Serum storage at -20°C provided adequate ROM stability for up to 3 months, whereas storage at 4°C yielded non-reproducible results. In conclusion, our data provide evidance that the D-ROM assay has both an acceptable stability and an adequate imprecision. The automated assay may be regarded as a fast and reproducible method for the quantitative evaluation of oxidative stress. Since it is easily performed, the method is suitable for routine in clinical laboratories and may provide an accurate estimation of oxidative stress in vivo.

Original languageEnglish
Pages (from-to)673-676
Number of pages4
JournalClinical Chemistry and Laboratory Medicine
Volume40
Issue number7
Publication statusPublished - 2002

Fingerprint

Metabolites
Assays
Oxygen
Oxidative stress
Serum
Oxidative Stress
Maximum Allowable Concentration
Clinical laboratories
Automation
Bilirubin
Organic compounds
Free Radicals
Half-Life
Hemoglobins
Triglycerides
Guidelines

Keywords

  • Free radicals
  • Hydrogen peroxide
  • Oxidative stress
  • Reactive oxygen metabolites

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Evaluation of an automated spectrophotometric assay for reactive oxygen metabolites in serum. / Iamele, Luigia; Fiocchi, Roberto; Vernocchi, Arialdo.

In: Clinical Chemistry and Laboratory Medicine, Vol. 40, No. 7, 2002, p. 673-676.

Research output: Contribution to journalArticle

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