Abstract
Background. Accurate estimation of haematopoietic stem cell (HSC) counts by flow cytometry may be difficult in laboratories in which sophisticated equipment and staff with specific expertise are not available. Affordable flow cytometers that can perform basic functions may help to overcome these difficulties. In this study we compared HSC and leucocyte counts determined by volumetric and bead-based protocols performed with the small, low-cost Accuri® C6, with those obtained with two gold-standard instruments, the four-colour FACSCalibur® and the eight-colour FACSCantoII®, our reference flow cytometers. Materials and methods. With the three cytometers we tested, in parallel, 111 consecutive samples from cord blood, peripheral blood from patients with myelofibrosis and myeloproliferative syndromes, fresh and thawed HSC collected by apheresis and bone marrow products. The findings were compared with one-way ANOVA, Bland-Altman analysis and linear regression. Results. The results of HSC and leucocyte enumeration by the three devices were strongly correlated (r2>0.99; p
| Original language | English |
|---|---|
| Pages (from-to) | 416-420 |
| Number of pages | 5 |
| Journal | Blood Transfusion |
| Volume | 12 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2014 |
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Keywords
- Apheresis
- Haematopoietic stem cells
- Volumetric flow cytometry
ASJC Scopus subject areas
- Hematology
- Immunology and Allergy
- Medicine(all)
Cite this
Evaluation of an easy and affordable flow cytometer for volumetric haematopoietic stem cell counting. / Mariani, Mariagabriella; Colombo, Federico; Assennato, Sonny M.; Frugoni, Cecilia; Cattaneo, Alessandra; Trombetta, Elena; Rebulla, Paolo; Porretti, Laura.
In: Blood Transfusion, Vol. 12, No. 3, 2014, p. 416-420.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Evaluation of an easy and affordable flow cytometer for volumetric haematopoietic stem cell counting
AU - Mariani, Mariagabriella
AU - Colombo, Federico
AU - Assennato, Sonny M.
AU - Frugoni, Cecilia
AU - Cattaneo, Alessandra
AU - Trombetta, Elena
AU - Rebulla, Paolo
AU - Porretti, Laura
PY - 2014
Y1 - 2014
N2 - Background. Accurate estimation of haematopoietic stem cell (HSC) counts by flow cytometry may be difficult in laboratories in which sophisticated equipment and staff with specific expertise are not available. Affordable flow cytometers that can perform basic functions may help to overcome these difficulties. In this study we compared HSC and leucocyte counts determined by volumetric and bead-based protocols performed with the small, low-cost Accuri® C6, with those obtained with two gold-standard instruments, the four-colour FACSCalibur® and the eight-colour FACSCantoII®, our reference flow cytometers. Materials and methods. With the three cytometers we tested, in parallel, 111 consecutive samples from cord blood, peripheral blood from patients with myelofibrosis and myeloproliferative syndromes, fresh and thawed HSC collected by apheresis and bone marrow products. The findings were compared with one-way ANOVA, Bland-Altman analysis and linear regression. Results. The results of HSC and leucocyte enumeration by the three devices were strongly correlated (r2>0.99; p
AB - Background. Accurate estimation of haematopoietic stem cell (HSC) counts by flow cytometry may be difficult in laboratories in which sophisticated equipment and staff with specific expertise are not available. Affordable flow cytometers that can perform basic functions may help to overcome these difficulties. In this study we compared HSC and leucocyte counts determined by volumetric and bead-based protocols performed with the small, low-cost Accuri® C6, with those obtained with two gold-standard instruments, the four-colour FACSCalibur® and the eight-colour FACSCantoII®, our reference flow cytometers. Materials and methods. With the three cytometers we tested, in parallel, 111 consecutive samples from cord blood, peripheral blood from patients with myelofibrosis and myeloproliferative syndromes, fresh and thawed HSC collected by apheresis and bone marrow products. The findings were compared with one-way ANOVA, Bland-Altman analysis and linear regression. Results. The results of HSC and leucocyte enumeration by the three devices were strongly correlated (r2>0.99; p
KW - Apheresis
KW - Haematopoietic stem cells
KW - Volumetric flow cytometry
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UR - http://www.scopus.com/inward/citedby.url?scp=84904644786&partnerID=8YFLogxK
U2 - 10.2450/2014.0198-13
DO - 10.2450/2014.0198-13
M3 - Article
C2 - 24887218
AN - SCOPUS:84904644786
VL - 12
SP - 416
EP - 420
JO - Blood Transfusion
JF - Blood Transfusion
SN - 1723-2007
IS - 3
ER -