Evaluation of an easy and affordable flow cytometer for volumetric haematopoietic stem cell counting

Mariagabriella Mariani, Federico Colombo, Sonny M. Assennato, Cecilia Frugoni, Alessandra Cattaneo, Elena Trombetta, Paolo Rebulla, Laura Porretti

Research output: Contribution to journalArticle

Abstract

Background. Accurate estimation of haematopoietic stem cell (HSC) counts by flow cytometry may be difficult in laboratories in which sophisticated equipment and staff with specific expertise are not available. Affordable flow cytometers that can perform basic functions may help to overcome these difficulties. In this study we compared HSC and leucocyte counts determined by volumetric and bead-based protocols performed with the small, low-cost Accuri® C6, with those obtained with two gold-standard instruments, the four-colour FACSCalibur® and the eight-colour FACSCantoII®, our reference flow cytometers. Materials and methods. With the three cytometers we tested, in parallel, 111 consecutive samples from cord blood, peripheral blood from patients with myelofibrosis and myeloproliferative syndromes, fresh and thawed HSC collected by apheresis and bone marrow products. The findings were compared with one-way ANOVA, Bland-Altman analysis and linear regression. Results. The results of HSC and leucocyte enumeration by the three devices were strongly correlated (r2>0.99; p

Original languageEnglish
Pages (from-to)416-420
Number of pages5
JournalBlood Transfusion
Volume12
Issue number3
DOIs
Publication statusPublished - 2014

Fingerprint

Hematopoietic Stem Cells
Color
Cell Count
Equipment and Supplies
Primary Myelofibrosis
Blood Component Removal
Fetal Blood
Leukocyte Count
Gold
Linear Models
Analysis of Variance
Flow Cytometry
Leukocytes
Bone Marrow
Costs and Cost Analysis

Keywords

  • Apheresis
  • Haematopoietic stem cells
  • Volumetric flow cytometry

ASJC Scopus subject areas

  • Hematology
  • Immunology and Allergy
  • Medicine(all)

Cite this

Evaluation of an easy and affordable flow cytometer for volumetric haematopoietic stem cell counting. / Mariani, Mariagabriella; Colombo, Federico; Assennato, Sonny M.; Frugoni, Cecilia; Cattaneo, Alessandra; Trombetta, Elena; Rebulla, Paolo; Porretti, Laura.

In: Blood Transfusion, Vol. 12, No. 3, 2014, p. 416-420.

Research output: Contribution to journalArticle

Mariani, Mariagabriella ; Colombo, Federico ; Assennato, Sonny M. ; Frugoni, Cecilia ; Cattaneo, Alessandra ; Trombetta, Elena ; Rebulla, Paolo ; Porretti, Laura. / Evaluation of an easy and affordable flow cytometer for volumetric haematopoietic stem cell counting. In: Blood Transfusion. 2014 ; Vol. 12, No. 3. pp. 416-420.
@article{33ffc665591849c7bb20186a621621e9,
title = "Evaluation of an easy and affordable flow cytometer for volumetric haematopoietic stem cell counting",
abstract = "Background. Accurate estimation of haematopoietic stem cell (HSC) counts by flow cytometry may be difficult in laboratories in which sophisticated equipment and staff with specific expertise are not available. Affordable flow cytometers that can perform basic functions may help to overcome these difficulties. In this study we compared HSC and leucocyte counts determined by volumetric and bead-based protocols performed with the small, low-cost Accuri{\circledR} C6, with those obtained with two gold-standard instruments, the four-colour FACSCalibur{\circledR} and the eight-colour FACSCantoII{\circledR}, our reference flow cytometers. Materials and methods. With the three cytometers we tested, in parallel, 111 consecutive samples from cord blood, peripheral blood from patients with myelofibrosis and myeloproliferative syndromes, fresh and thawed HSC collected by apheresis and bone marrow products. The findings were compared with one-way ANOVA, Bland-Altman analysis and linear regression. Results. The results of HSC and leucocyte enumeration by the three devices were strongly correlated (r2>0.99; p",
keywords = "Apheresis, Haematopoietic stem cells, Volumetric flow cytometry",
author = "Mariagabriella Mariani and Federico Colombo and Assennato, {Sonny M.} and Cecilia Frugoni and Alessandra Cattaneo and Elena Trombetta and Paolo Rebulla and Laura Porretti",
year = "2014",
doi = "10.2450/2014.0198-13",
language = "English",
volume = "12",
pages = "416--420",
journal = "Blood Transfusion",
issn = "1723-2007",
publisher = "Edizioni SIMTI",
number = "3",

}

TY - JOUR

T1 - Evaluation of an easy and affordable flow cytometer for volumetric haematopoietic stem cell counting

AU - Mariani, Mariagabriella

AU - Colombo, Federico

AU - Assennato, Sonny M.

AU - Frugoni, Cecilia

AU - Cattaneo, Alessandra

AU - Trombetta, Elena

AU - Rebulla, Paolo

AU - Porretti, Laura

PY - 2014

Y1 - 2014

N2 - Background. Accurate estimation of haematopoietic stem cell (HSC) counts by flow cytometry may be difficult in laboratories in which sophisticated equipment and staff with specific expertise are not available. Affordable flow cytometers that can perform basic functions may help to overcome these difficulties. In this study we compared HSC and leucocyte counts determined by volumetric and bead-based protocols performed with the small, low-cost Accuri® C6, with those obtained with two gold-standard instruments, the four-colour FACSCalibur® and the eight-colour FACSCantoII®, our reference flow cytometers. Materials and methods. With the three cytometers we tested, in parallel, 111 consecutive samples from cord blood, peripheral blood from patients with myelofibrosis and myeloproliferative syndromes, fresh and thawed HSC collected by apheresis and bone marrow products. The findings were compared with one-way ANOVA, Bland-Altman analysis and linear regression. Results. The results of HSC and leucocyte enumeration by the three devices were strongly correlated (r2>0.99; p

AB - Background. Accurate estimation of haematopoietic stem cell (HSC) counts by flow cytometry may be difficult in laboratories in which sophisticated equipment and staff with specific expertise are not available. Affordable flow cytometers that can perform basic functions may help to overcome these difficulties. In this study we compared HSC and leucocyte counts determined by volumetric and bead-based protocols performed with the small, low-cost Accuri® C6, with those obtained with two gold-standard instruments, the four-colour FACSCalibur® and the eight-colour FACSCantoII®, our reference flow cytometers. Materials and methods. With the three cytometers we tested, in parallel, 111 consecutive samples from cord blood, peripheral blood from patients with myelofibrosis and myeloproliferative syndromes, fresh and thawed HSC collected by apheresis and bone marrow products. The findings were compared with one-way ANOVA, Bland-Altman analysis and linear regression. Results. The results of HSC and leucocyte enumeration by the three devices were strongly correlated (r2>0.99; p

KW - Apheresis

KW - Haematopoietic stem cells

KW - Volumetric flow cytometry

UR - http://www.scopus.com/inward/record.url?scp=84904644786&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84904644786&partnerID=8YFLogxK

U2 - 10.2450/2014.0198-13

DO - 10.2450/2014.0198-13

M3 - Article

C2 - 24887218

AN - SCOPUS:84904644786

VL - 12

SP - 416

EP - 420

JO - Blood Transfusion

JF - Blood Transfusion

SN - 1723-2007

IS - 3

ER -