Evaluation of basophil activation test in suspected food hypersensitivity

Patrizia Pignatti, Mona Rita Yacoub, Claudia Testoni, Gianni Pala, Maura Corsetti, Giselda Colombo, Antonio Meriggi, Gianna Moscato

Research output: Contribution to journalArticle

Abstract

Background: Food hypersensitivity is characterized by a wide range of symptoms. The relationship between symptoms and food is more frequently suspected than objectively proven. Basophil activation test (BAT) is based on the evaluation of activation markers on blood basophils in vitro stimulated with drugs or allergens. The aim of the study was to evaluate the usefulness of BAT when introduced in the routine work-up of suspected food hypersensitivity. Methods: BAT was requested in subjects with food adverse reactions when a discrepancy existed among history and skin prick test (SPT) and/or specific IgE. Data from 150 subjects were analysed using CD63 as basophil activation marker. Thirty controls were evaluated for cut-offs. Immunoblots was performed with the sera of representative subjects positive for BAT and negative for SPT and sIgE. Results: 1,024 BAT were carried out, the agreement (positive/positive and negative/negative) was 78.5% for BAT vs. SPT and 78.3% for BAT vs. IgE. Atopic patients, but not atopic controls, more frequently had a positive BAT than non-atopic patients (P

Original languageEnglish
Pages (from-to)279-285
JournalCytometry Part B - Clinical Cytometry
DOIs
Publication statusPublished - Jul 2017

Keywords

  • Basophil activation test
  • CD63
  • Food hypersensitivity

ASJC Scopus subject areas

  • Cell Biology
  • Histology
  • Pathology and Forensic Medicine

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