Evaluation of cardiovascular risk factors in the Wistar Audiogenic Rat (WAR) strain

Rubens Fazan, Carlos Alberto A Silva, José Antônio Cortes Oliveira, Helio Cesar Salgado, Nicola Montano, Norberto Garcia-Cairasco

Research output: Contribution to journalArticle

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Abstract

Introduction: Risk factors for life-threatening cardiovascular events were evaluated in an experimental model of epilepsy, the Wistar Audiogenic Rat (WAR) strain. Methods: We used long-term ECG recordings in conscious, one year old, WAR and Wistar control counterparts to evaluate spontaneous arrhythmias and heart rate variability, a tool to assess autonomic cardiac control. Ventricular function was also evaluated using the pressure-volume conductance system in anesthetized rats. Results: Basal RR interval (RRi) was similar between WAR and Wistar rats (188±5 vs 199±6 ms). RRi variability strongly suggests that WAR present an autonomic imbalance with sympathetic overactivity, which is an isolated risk factor for cardiovascular events. Anesthetized WAR showed lower arterial pressure (92±3 vs 115±5 mmHg) and exhibited indices of systolic dysfunction, such as higher ventricle end-diastolic pressure (9.2±0.6 vs 5.6±1 mmHg) and volume (137±9 vs 68±9 μL) as well as lower rate of increase in ventricular pressure (5266±602 vs 7320±538 mmHg.s-1). Indices of diastolic cardiac function, such as lower rate of decrease in ventricular pressure (-5014±780 vs -7766±998 mmHg.s-1) and a higher slope of the linear relationship between end-diastolic pressure and volume (0.078±0.011 vs 0.036±0.011 mmHg.μL), were also found in WAR as compared to Wistar control rats. Moreover, Wistar rats had 3 to 6 ventricular ectopic beats, whereas WAR showed 15 to 30 ectopic beats out of the 20,000 beats analyzed in each rat. Conclusions: The autonomic imbalance observed previously at younger age is also present in aged WAR and, additionally, a cardiac dysfunction was also observed in the rats. These findings make this experimental model of epilepsy a valuable tool to study risk factors for cardiovascular events in epilepsy.

Original languageEnglish
Article numbere0129574
JournalPLoS One
Volume10
Issue number6
DOIs
Publication statusPublished - Jun 1 2015

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Wistar Rats
Rats
risk factors
rats
epilepsy
Ventricular Pressure
Epilepsy
Theoretical Models
Reflex Epilepsy
Blood Pressure
Rat control
Ventricular Premature Complexes
Ventricular Function
arrhythmia
Cardiac Arrhythmias
Electrocardiography
cardiac output
Arterial Pressure
Heart Rate
heart rate

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Fazan, R., Silva, C. A. A., Oliveira, J. A. C., Salgado, H. C., Montano, N., & Garcia-Cairasco, N. (2015). Evaluation of cardiovascular risk factors in the Wistar Audiogenic Rat (WAR) strain. PLoS One, 10(6), [e0129574]. https://doi.org/10.1371/journal.pone.0129574

Evaluation of cardiovascular risk factors in the Wistar Audiogenic Rat (WAR) strain. / Fazan, Rubens; Silva, Carlos Alberto A; Oliveira, José Antônio Cortes; Salgado, Helio Cesar; Montano, Nicola; Garcia-Cairasco, Norberto.

In: PLoS One, Vol. 10, No. 6, e0129574, 01.06.2015.

Research output: Contribution to journalArticle

Fazan, R, Silva, CAA, Oliveira, JAC, Salgado, HC, Montano, N & Garcia-Cairasco, N 2015, 'Evaluation of cardiovascular risk factors in the Wistar Audiogenic Rat (WAR) strain', PLoS One, vol. 10, no. 6, e0129574. https://doi.org/10.1371/journal.pone.0129574
Fazan, Rubens ; Silva, Carlos Alberto A ; Oliveira, José Antônio Cortes ; Salgado, Helio Cesar ; Montano, Nicola ; Garcia-Cairasco, Norberto. / Evaluation of cardiovascular risk factors in the Wistar Audiogenic Rat (WAR) strain. In: PLoS One. 2015 ; Vol. 10, No. 6.
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