Evaluation of chemokine and cytokine profiles in osteoblast progenitors from umbilical cord blood stem cells by BIO-PLEX technology

Letizia Penolazzi, Elisabetta Lambertini, Elisa Tavanti, Elena Torreggiani, Fortunato Vesce, Roberto Gambari, Roberta Piva

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

We have used cytokine protein array to analyze the secretion of cytokines from an osteoblastic clone derived from human umbilical cord blood mesenchymal stem cells (MSCs) cultured in an osteogenic differentiation medium. The analysis demonstrated the unexpected ability of osteoblast committed cells and their early progenitors to produce significant amounts of a range of soluble immune mediators without in vitro exposure to clinically relevant bacterial pathogens. The cells were expanded and their osteogenic potential analyzed over 45 days of culture was revealed by the expression of osteoblast-specific markers (alkaline phosphatase and Runx2), and by matrix mineralization. Over this culture period, the cells secreted particularly high levels of IL-8, MCP-1 and VEGF, but did not express IL-2, IL-7, IL-17, eotaxin, G-CSF and IFN-γ. These findings should encourage the use of human umbilical cord blood as a potential stem cells source for bone regeneration.

Original languageEnglish
Pages (from-to)320-325
Number of pages6
JournalCell Biology International
Volume32
Issue number2
DOIs
Publication statusPublished - Feb 2008

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Osteoblasts
Fetal Blood
Chemokines
Blood Cells
Stem Cells
Cytokines
Technology
Interleukin-7
Protein Array Analysis
Bone Regeneration
Interleukin-17
Granulocyte Colony-Stimulating Factor
Mesenchymal Stromal Cells
Interleukin-8
Vascular Endothelial Growth Factor A
Interleukin-2
Alkaline Phosphatase
Clone Cells

Keywords

  • Bio-plex analysis
  • Chemokines
  • Cytokines
  • Mesenchymal stem cells
  • Osteoblasts

ASJC Scopus subject areas

  • Cell Biology

Cite this

Evaluation of chemokine and cytokine profiles in osteoblast progenitors from umbilical cord blood stem cells by BIO-PLEX technology. / Penolazzi, Letizia; Lambertini, Elisabetta; Tavanti, Elisa; Torreggiani, Elena; Vesce, Fortunato; Gambari, Roberto; Piva, Roberta.

In: Cell Biology International, Vol. 32, No. 2, 02.2008, p. 320-325.

Research output: Contribution to journalArticle

Penolazzi, Letizia ; Lambertini, Elisabetta ; Tavanti, Elisa ; Torreggiani, Elena ; Vesce, Fortunato ; Gambari, Roberto ; Piva, Roberta. / Evaluation of chemokine and cytokine profiles in osteoblast progenitors from umbilical cord blood stem cells by BIO-PLEX technology. In: Cell Biology International. 2008 ; Vol. 32, No. 2. pp. 320-325.
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