Evaluation of circulating levels and renal clearance of natural amino acids in patients with Cushing's disease

A. Faggiano, R. Pivonello, D. Melis, R. Alfieri, M. Filippella, G. Spagnuolo, F. Salvatore, G. Lombardi, Annamaria Colao

Research output: Contribution to journalArticle

Abstract

Although the hypercortisolism-induced impairment of protein homeostasis is object of several studies, a detailed evaluation of the complete amino acid profile of patients with Cushing's syndrome (CS) has never been performed. The aim of the current open transversal controlled study was to evaluate serum and urinary concentrations as well as renal clearance of the complete series of natural amino acids and their relationship with glucose tolerance in patients with Cushing's disease (CD). Twenty patients with CD (10 active and 10 cured) and 20 sex- and age-matched healthy controls entered the study. Measurement of serum and urinary levels of the complete series of natural amino acids was performed in all patients analyzed by cationic exchange high performance liquid cromatography (HPLC) after 2 weeks of a standardized protein intake regimen. The renal clearance (renal excretion rate) of each amino acid was calculated on the basis of the serum and urinary concentrations of creatinine and the specific amino acid. Fasting glucose and insulin levels, glucose and insulin response to standard glucose load, insulinogenic and homeostasis model insulin resistance (Homa-R) indexes were also evaluated and correlated to the circulating levels and renal clearances of each amino acid. Significantly higher serum (p

Original languageEnglish
Pages (from-to)142-151
Number of pages10
JournalJournal of Endocrinological Investigation
Volume25
Issue number2
Publication statusPublished - 2002

Fingerprint

Pituitary ACTH Hypersecretion
Kidney
Amino Acids
Glucose
Cushing Syndrome
Serum
Homeostasis
Insulin
Insulin Resistance
Fasting
Creatinine
Proteins

Keywords

  • Amino acid metabolism
  • Cushing's disease
  • Hypercortisolism
  • Hyperglicemia
  • Insulin resistance
  • Obesity
  • Protein metabolism
  • Renal clearance

ASJC Scopus subject areas

  • Endocrinology

Cite this

Faggiano, A., Pivonello, R., Melis, D., Alfieri, R., Filippella, M., Spagnuolo, G., ... Colao, A. (2002). Evaluation of circulating levels and renal clearance of natural amino acids in patients with Cushing's disease. Journal of Endocrinological Investigation, 25(2), 142-151.

Evaluation of circulating levels and renal clearance of natural amino acids in patients with Cushing's disease. / Faggiano, A.; Pivonello, R.; Melis, D.; Alfieri, R.; Filippella, M.; Spagnuolo, G.; Salvatore, F.; Lombardi, G.; Colao, Annamaria.

In: Journal of Endocrinological Investigation, Vol. 25, No. 2, 2002, p. 142-151.

Research output: Contribution to journalArticle

Faggiano, A, Pivonello, R, Melis, D, Alfieri, R, Filippella, M, Spagnuolo, G, Salvatore, F, Lombardi, G & Colao, A 2002, 'Evaluation of circulating levels and renal clearance of natural amino acids in patients with Cushing's disease', Journal of Endocrinological Investigation, vol. 25, no. 2, pp. 142-151.
Faggiano A, Pivonello R, Melis D, Alfieri R, Filippella M, Spagnuolo G et al. Evaluation of circulating levels and renal clearance of natural amino acids in patients with Cushing's disease. Journal of Endocrinological Investigation. 2002;25(2):142-151.
Faggiano, A. ; Pivonello, R. ; Melis, D. ; Alfieri, R. ; Filippella, M. ; Spagnuolo, G. ; Salvatore, F. ; Lombardi, G. ; Colao, Annamaria. / Evaluation of circulating levels and renal clearance of natural amino acids in patients with Cushing's disease. In: Journal of Endocrinological Investigation. 2002 ; Vol. 25, No. 2. pp. 142-151.
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