Evaluation of different plasmid DNA delivery systems for immunization against HER2/neu in a transgenic murine model of mammary carcinoma

Arianna Smorlesi; Francesca Papalini; Augusto Amici; Fiorenza Orlando; Sara Pierpaoli; Chiara Mancini; Mauro Provinciali

doi:10.1016/j.vaccine.2005.10.022

Evaluation of different plasmid DNA delivery systems for immunization against HER2/neu in a transgenic murine model of mammary carcinoma

Arianna Smorlesi, Francesca Papalini, Augusto Amici, Fiorenza Orlando, Sara Pierpaoli, Chiara Mancini, Mauro Provinciali

Istituto Nazionale di Riposo e Cura per Anziani V.E. II

Research output: Contribution to journal › Article › peer-review

Abstract

Studies of DNA vaccination against HER2/neu showed the effectiveness of immunization protocols in models of transplantable or spontaneous tumors; scarce information, however, has been provided to identify the procedure of DNA administration that more effectively contributes to the activation of immune system against spontaneously arising HER2/neu-positive tumors. We compared the effectiveness of different procedures of DNA vaccine delivery (intradermic injection (ID), gene gun (GG) delivery and intramuscular injection (IM) alone or with electroporation) in a murine transgenic model of mammary carcinoma overexpressing HER2/neu. We highlighted the role of DNA delivery system in the success of DNA vaccination showing that, among the analysed methods, intramuscular injection of the vaccine, particularly when associated to electroporation, elicits a better protection against HER2/neu spontaneous tumor development inducing antibody and cell-mediated immune responsiveness against HER2/neu and a Th1 polarization of the immune response.

Original language	English
Pages (from-to)	1766-1775
Number of pages	10
Journal	Vaccine
Volume	24
Issue number	11
DOIs	https://doi.org/10.1016/j.vaccine.2005.10.022
Publication status	Published - Mar 10 2006

Keywords

DNA delivery system
DNA vaccination
HER2/neu
Immune response
Mammary tumor

ASJC Scopus subject areas

Immunology
Microbiology
Virology
Infectious Diseases
Public Health, Environmental and Occupational Health
veterinary(all)

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Evaluation of different plasmid DNA delivery systems for immunization against HER2/neu in a transgenic murine model of mammary carcinoma. / Smorlesi, Arianna; Papalini, Francesca; Amici, Augusto; Orlando, Fiorenza; Pierpaoli, Sara; Mancini, Chiara; Provinciali, Mauro.

In: Vaccine, Vol. 24, No. 11, 10.03.2006, p. 1766-1775.

Research output: Contribution to journal › Article › peer-review

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AU - Pierpaoli, Sara

AU - Mancini, Chiara

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