Evaluation of different plasmid DNA delivery systems for immunization against HER2/neu in a transgenic murine model of mammary carcinoma

Arianna Smorlesi, Francesca Papalini, Augusto Amici, Fiorenza Orlando, Sara Pierpaoli, Chiara Mancini, Mauro Provinciali

Research output: Contribution to journalArticle

Abstract

Studies of DNA vaccination against HER2/neu showed the effectiveness of immunization protocols in models of transplantable or spontaneous tumors; scarce information, however, has been provided to identify the procedure of DNA administration that more effectively contributes to the activation of immune system against spontaneously arising HER2/neu-positive tumors. We compared the effectiveness of different procedures of DNA vaccine delivery (intradermic injection (ID), gene gun (GG) delivery and intramuscular injection (IM) alone or with electroporation) in a murine transgenic model of mammary carcinoma overexpressing HER2/neu. We highlighted the role of DNA delivery system in the success of DNA vaccination showing that, among the analysed methods, intramuscular injection of the vaccine, particularly when associated to electroporation, elicits a better protection against HER2/neu spontaneous tumor development inducing antibody and cell-mediated immune responsiveness against HER2/neu and a Th1 polarization of the immune response.

Original languageEnglish
Pages (from-to)1766-1775
Number of pages10
JournalVaccine
Volume24
Issue number11
DOIs
Publication statusPublished - Mar 10 2006

Keywords

  • DNA delivery system
  • DNA vaccination
  • HER2/neu
  • Immune response
  • Mammary tumor

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

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