Evaluation of Elisa test for therapeutic monitoring of Nelfinavir in HIV-positive patients

Alessia Uglietti, Francesca Genco, Elena Donadel, Serena Rinaldi, Erica Bastiani, Renato Maserati, Valeria Meroni

Research output: Contribution to journalArticle

Abstract

Therapeutic drug monitoring (TDM) is an important tool in the management of antiretroviral (ARV) therapy.The gold standard for measuring drugs plasma levels is High-Performance Liquid Chromatographic Assay (HPLC) however it is technically-demanding and time-consuming. We evaluated a new immunoenzymatic test (TDM-ELISA®, Biostrands, Trieste, Italy) for nelfinavir and its active metabolite M8 in comparison with HPLC. A statistically significant difference in Ctrough between the two different tests was demonstrated but this difference was no longer significant when a value of 29% due to M8 aliquot was deleted. This faster TDM-ELISA® may have an important role for TDM in HIV patients taking ARVs.

Original languageEnglish
Pages (from-to)318-320
Number of pages3
JournalNew Microbiologica
Volume30
Issue number3
Publication statusPublished - Jul 2007

Keywords

  • Nelfinavir
  • TDM-ELISA
  • Therapeutic drug monitoring

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

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