Evaluation of gene expression in human lymphocytes activated in the presence of melatonin

Enrica Capelli, Ilaria Campo, Simona Panelli, Giuseppe Damiani, Maria Grazia Santagostino Barbone, Adele Lucchelli, Mariaclara Cuccia

Research output: Contribution to journalArticle

Abstract

The effect of melatonin on the expression of genes previously correlated to T lymphocyte activation (HLA-DRB, thymosin beta 10 (β-Tim)) and to Lymphokine Activated Killer (LAK) activity (β-Tim, Tumour Rejection Antigen (TRA 1), nRap 2) was investigated in phytohemagglutinin (PHA)-stimulated human lymphocyte cultures. The aim was to find an enhancing effect of this substance on anti-tumoral immune defences as suggested by studies on tumour progression in mice and clinical immunotherapy trials in humans. mRNA obtained from melatonin-treated and -untreated PHA-stimulated lymphocytes was retrotranscribed and amplified by RT-PCR using primers based on the sequences of the selected genes. The results suggest that melatonin does not increase T and LAK cell responses: in fact, a reduction in the transcription of all the considered genes was observed. These data are correlated with the antiproliferative effect of melatonin observed in in vitro treated lymphocytes.

Original languageEnglish
Pages (from-to)885-892
Number of pages8
JournalInternational Immunopharmacology
Volume2
Issue number7
DOIs
Publication statusPublished - 2002

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Keywords

  • LAK cells
  • Melatonin
  • T lymphocytes

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

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