Evaluation of genome-wide expression profiles of blood and sputum neutrophils in cystic fibrosis patients before and after antibiotic therapy

Massimo Conese, Stefano Castellani, Silvia Lepore, Orazio Palumbo, Antonio Manca, Teresa Santostasi, Angela Maria Polizzi, Massimiliano Copetti, Sante Di Gioia, Valeria Casavola, Lorenzo Guerra, Anna Diana, Pasqualina Montemurro, Maria Addolorata Mariggiò, Crescenzio Gallo, Angela Bruna Maffione, Massimo Carella

Research output: Contribution to journalArticle

Abstract

In seeking more specific biomarkers of the cystic fibrosis (CF) lung inflammatory disease that would be sensitive to antibiotic therapy, we sought to evaluate the gene expression profiles of neutrophils in CF patients before treatment in comparison with non-CF healthy individuals and after antibiotic treatment. Genes involved in neutrophil-mediated inflammation, i.e. chemotaxis, respiratory burst, apoptosis, and granule exocytosis, were the targets of this study. Microarray analysis was carried out in blood and airway neutrophils from CF patients and in control subjects. A fold change (log) threshold of 1.4 and a cut-off of p

Original languageEnglish
Article numbere104080
JournalPLoS One
Volume9
Issue number8
DOIs
Publication statusPublished - Aug 1 2014

    Fingerprint

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Conese, M., Castellani, S., Lepore, S., Palumbo, O., Manca, A., Santostasi, T., Polizzi, A. M., Copetti, M., Di Gioia, S., Casavola, V., Guerra, L., Diana, A., Montemurro, P., Mariggiò, M. A., Gallo, C., Maffione, A. B., & Carella, M. (2014). Evaluation of genome-wide expression profiles of blood and sputum neutrophils in cystic fibrosis patients before and after antibiotic therapy. PLoS One, 9(8), [e104080]. https://doi.org/10.1371/journal.pone.0104080