Evaluation of immune activation in HIV-infected and uninfected African individuals by single-cell analysis of cytokine production

Matthew Lukwiya, Giuliano Rizzardini, Daria Trabattoni, Stefania Piconi, Marina Saresella, Silvia Declich, Sabrina Fossati, Mario Clerici

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Immune activation has been observed in HIV-infected and uninfected Africans, among whom it is thought to modify interaction between the immune system and HIV. To characterize this phenomenon accurately, in-depth immunologic analyses were performed in a rural African population. Freshly drawn peripheral blood mononuclear cells (PBMCs) of HIV-infected African (from Gulu, Uganda) and Italian antiviral-naive patients and those of uninfected Ugandan and Italian study subjects were analyzed. Individuals were matched for age and sex and determined to be free from parasitic infections. Intracellular cytokines were measured in mitogen (M)- and gp160 peptides + staphylococcal enterotoxin B and αCD28 (env)-stimulated T lymphocytes. Interferon (IFN)-γ-producing CD8+ T cells were quantified in an enzyme-linked immunosorbent assay. Results showed that M-stimulated production of interleukin (IL)-10 and tumor necrosis factor (TNF)-α increases in CD4+ and CD8+ cells of African infected patients and uninfected study subject; and that env-stimulated IL-10 and TNF-α production is increased in CD8+ T lymphocytes of African HIV-infected patients. M- and env-stimulated IFN-γ-producing CD8+ T cells were reduced in African participants and not increased by preincubation with αIL-10 monoclonal antibody. This is the first set of data that has reported immune activation in rural Africa by single-cell analysis of cytokine production. These results help in defining the immunologic background to be considered in the design of therapeutic and vaccine-based approaches to HIV infection in an African setting.

Original languageEnglish
Pages (from-to)429-436
Number of pages8
JournalJournal of Acquired Immune Deficiency Syndromes
Volume28
Issue number5
Publication statusPublished - Dec 15 2001

Fingerprint

Single-Cell Analysis
HIV
Interleukin-10
Cytokines
T-Lymphocytes
Interferons
Tumor Necrosis Factor-alpha
Parasitic Diseases
Uganda
Rural Population
Mitogens
HIV Infections
Antiviral Agents
Immune System
Blood Cells
Vaccines
Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies

Keywords

  • Africa
  • Cytokine
  • HIV infection
  • Immune activation
  • Immunity

ASJC Scopus subject areas

  • Virology
  • Immunology

Cite this

Evaluation of immune activation in HIV-infected and uninfected African individuals by single-cell analysis of cytokine production. / Lukwiya, Matthew; Rizzardini, Giuliano; Trabattoni, Daria; Piconi, Stefania; Saresella, Marina; Declich, Silvia; Fossati, Sabrina; Clerici, Mario.

In: Journal of Acquired Immune Deficiency Syndromes, Vol. 28, No. 5, 15.12.2001, p. 429-436.

Research output: Contribution to journalArticle

Lukwiya, Matthew ; Rizzardini, Giuliano ; Trabattoni, Daria ; Piconi, Stefania ; Saresella, Marina ; Declich, Silvia ; Fossati, Sabrina ; Clerici, Mario. / Evaluation of immune activation in HIV-infected and uninfected African individuals by single-cell analysis of cytokine production. In: Journal of Acquired Immune Deficiency Syndromes. 2001 ; Vol. 28, No. 5. pp. 429-436.
@article{558e2fe5e4744622b9fbbdb9a8a2ae48,
title = "Evaluation of immune activation in HIV-infected and uninfected African individuals by single-cell analysis of cytokine production",
abstract = "Immune activation has been observed in HIV-infected and uninfected Africans, among whom it is thought to modify interaction between the immune system and HIV. To characterize this phenomenon accurately, in-depth immunologic analyses were performed in a rural African population. Freshly drawn peripheral blood mononuclear cells (PBMCs) of HIV-infected African (from Gulu, Uganda) and Italian antiviral-naive patients and those of uninfected Ugandan and Italian study subjects were analyzed. Individuals were matched for age and sex and determined to be free from parasitic infections. Intracellular cytokines were measured in mitogen (M)- and gp160 peptides + staphylococcal enterotoxin B and αCD28 (env)-stimulated T lymphocytes. Interferon (IFN)-γ-producing CD8+ T cells were quantified in an enzyme-linked immunosorbent assay. Results showed that M-stimulated production of interleukin (IL)-10 and tumor necrosis factor (TNF)-α increases in CD4+ and CD8+ cells of African infected patients and uninfected study subject; and that env-stimulated IL-10 and TNF-α production is increased in CD8+ T lymphocytes of African HIV-infected patients. M- and env-stimulated IFN-γ-producing CD8+ T cells were reduced in African participants and not increased by preincubation with αIL-10 monoclonal antibody. This is the first set of data that has reported immune activation in rural Africa by single-cell analysis of cytokine production. These results help in defining the immunologic background to be considered in the design of therapeutic and vaccine-based approaches to HIV infection in an African setting.",
keywords = "Africa, Cytokine, HIV infection, Immune activation, Immunity",
author = "Matthew Lukwiya and Giuliano Rizzardini and Daria Trabattoni and Stefania Piconi and Marina Saresella and Silvia Declich and Sabrina Fossati and Mario Clerici",
year = "2001",
month = "12",
day = "15",
language = "English",
volume = "28",
pages = "429--436",
journal = "Journal of Acquired Immune Deficiency Syndromes",
issn = "1525-4135",
publisher = "Lippincott Williams and Wilkins",
number = "5",

}

TY - JOUR

T1 - Evaluation of immune activation in HIV-infected and uninfected African individuals by single-cell analysis of cytokine production

AU - Lukwiya, Matthew

AU - Rizzardini, Giuliano

AU - Trabattoni, Daria

AU - Piconi, Stefania

AU - Saresella, Marina

AU - Declich, Silvia

AU - Fossati, Sabrina

AU - Clerici, Mario

PY - 2001/12/15

Y1 - 2001/12/15

N2 - Immune activation has been observed in HIV-infected and uninfected Africans, among whom it is thought to modify interaction between the immune system and HIV. To characterize this phenomenon accurately, in-depth immunologic analyses were performed in a rural African population. Freshly drawn peripheral blood mononuclear cells (PBMCs) of HIV-infected African (from Gulu, Uganda) and Italian antiviral-naive patients and those of uninfected Ugandan and Italian study subjects were analyzed. Individuals were matched for age and sex and determined to be free from parasitic infections. Intracellular cytokines were measured in mitogen (M)- and gp160 peptides + staphylococcal enterotoxin B and αCD28 (env)-stimulated T lymphocytes. Interferon (IFN)-γ-producing CD8+ T cells were quantified in an enzyme-linked immunosorbent assay. Results showed that M-stimulated production of interleukin (IL)-10 and tumor necrosis factor (TNF)-α increases in CD4+ and CD8+ cells of African infected patients and uninfected study subject; and that env-stimulated IL-10 and TNF-α production is increased in CD8+ T lymphocytes of African HIV-infected patients. M- and env-stimulated IFN-γ-producing CD8+ T cells were reduced in African participants and not increased by preincubation with αIL-10 monoclonal antibody. This is the first set of data that has reported immune activation in rural Africa by single-cell analysis of cytokine production. These results help in defining the immunologic background to be considered in the design of therapeutic and vaccine-based approaches to HIV infection in an African setting.

AB - Immune activation has been observed in HIV-infected and uninfected Africans, among whom it is thought to modify interaction between the immune system and HIV. To characterize this phenomenon accurately, in-depth immunologic analyses were performed in a rural African population. Freshly drawn peripheral blood mononuclear cells (PBMCs) of HIV-infected African (from Gulu, Uganda) and Italian antiviral-naive patients and those of uninfected Ugandan and Italian study subjects were analyzed. Individuals were matched for age and sex and determined to be free from parasitic infections. Intracellular cytokines were measured in mitogen (M)- and gp160 peptides + staphylococcal enterotoxin B and αCD28 (env)-stimulated T lymphocytes. Interferon (IFN)-γ-producing CD8+ T cells were quantified in an enzyme-linked immunosorbent assay. Results showed that M-stimulated production of interleukin (IL)-10 and tumor necrosis factor (TNF)-α increases in CD4+ and CD8+ cells of African infected patients and uninfected study subject; and that env-stimulated IL-10 and TNF-α production is increased in CD8+ T lymphocytes of African HIV-infected patients. M- and env-stimulated IFN-γ-producing CD8+ T cells were reduced in African participants and not increased by preincubation with αIL-10 monoclonal antibody. This is the first set of data that has reported immune activation in rural Africa by single-cell analysis of cytokine production. These results help in defining the immunologic background to be considered in the design of therapeutic and vaccine-based approaches to HIV infection in an African setting.

KW - Africa

KW - Cytokine

KW - HIV infection

KW - Immune activation

KW - Immunity

UR - http://www.scopus.com/inward/record.url?scp=0035894220&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035894220&partnerID=8YFLogxK

M3 - Article

VL - 28

SP - 429

EP - 436

JO - Journal of Acquired Immune Deficiency Syndromes

JF - Journal of Acquired Immune Deficiency Syndromes

SN - 1525-4135

IS - 5

ER -