Evaluation of in Vivo Response of Three Biphasic Scaffolds for Osteochondral Tissue Regeneration in a Sheep Model

Alberto M Crovace, Alessia Di Giancamillo, Francesca Gervaso, Laura Mangiavini, Davide Zani, Francesca Scalera, Barbara Palazzo, Daniela Izzo, Marco Agnoletto, Marco Domenicucci, Corrado Sosio, Alessandro Sannino, Mauro Di Giancamillo, Giuseppe M Peretti

Research output: Contribution to journalArticle

Abstract

Osteochondral defects are a common problem in both human medicine and veterinary practice although with important limits concerning the cartilaginous tissue regeneration. Interest in the subchondral bone has grown, as it is now considered a key element in the osteochondral defect healing. The aim of this work was to generate and to evaluate the architecture of three cell-free scaffolds made of collagen, magnesium/hydroxyapatite and collagen hydroxyapatite/wollastonite to be implanted in a sheep animal model. Scaffolds were designed in a bilayer configuration and a novel "Honey" configuration, where columns of hydroxyapatite were inserted within the collagen matrix. The use of different types of scaffolds allowed us to identify the best scaffold in terms of integration and tissue regeneration. The animals included were divided into four groups: three were treated using different types of scaffold while one was left untreated and represented the control group. Evaluations were made at 3 months through CT analysis. The novel "Honey" configuration of the scaffold with hydroxyapatite seems to allow for a better reparative process, although we are still far from obtaining a complete restoration of the defect at this time point of follow-up.

Original languageEnglish
JournalVeterinary Sciences
Volume6
Issue number4
DOIs
Publication statusPublished - Nov 9 2019

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Tissue Scaffolds
hydroxyapatite
Durapatite
tissue repair
Regeneration
Sheep
collagen
sheep
Collagen
Honey
honey
calcium silicate
Veterinary Medicine
Magnesium
magnesium
medicine
Animal Models
animal models
bones
Bone and Bones

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Evaluation of in Vivo Response of Three Biphasic Scaffolds for Osteochondral Tissue Regeneration in a Sheep Model. / Crovace, Alberto M; Giancamillo, Alessia Di; Gervaso, Francesca; Mangiavini, Laura; Zani, Davide; Scalera, Francesca; Palazzo, Barbara; Izzo, Daniela; Agnoletto, Marco; Domenicucci, Marco; Sosio, Corrado; Sannino, Alessandro; Giancamillo, Mauro Di; Peretti, Giuseppe M.

In: Veterinary Sciences, Vol. 6, No. 4, 09.11.2019.

Research output: Contribution to journalArticle

Crovace, AM, Giancamillo, AD, Gervaso, F, Mangiavini, L, Zani, D, Scalera, F, Palazzo, B, Izzo, D, Agnoletto, M, Domenicucci, M, Sosio, C, Sannino, A, Giancamillo, MD & Peretti, GM 2019, 'Evaluation of in Vivo Response of Three Biphasic Scaffolds for Osteochondral Tissue Regeneration in a Sheep Model', Veterinary Sciences, vol. 6, no. 4. https://doi.org/10.3390/vetsci6040090
Crovace, Alberto M ; Giancamillo, Alessia Di ; Gervaso, Francesca ; Mangiavini, Laura ; Zani, Davide ; Scalera, Francesca ; Palazzo, Barbara ; Izzo, Daniela ; Agnoletto, Marco ; Domenicucci, Marco ; Sosio, Corrado ; Sannino, Alessandro ; Giancamillo, Mauro Di ; Peretti, Giuseppe M. / Evaluation of in Vivo Response of Three Biphasic Scaffolds for Osteochondral Tissue Regeneration in a Sheep Model. In: Veterinary Sciences. 2019 ; Vol. 6, No. 4.
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AB - Osteochondral defects are a common problem in both human medicine and veterinary practice although with important limits concerning the cartilaginous tissue regeneration. Interest in the subchondral bone has grown, as it is now considered a key element in the osteochondral defect healing. The aim of this work was to generate and to evaluate the architecture of three cell-free scaffolds made of collagen, magnesium/hydroxyapatite and collagen hydroxyapatite/wollastonite to be implanted in a sheep animal model. Scaffolds were designed in a bilayer configuration and a novel "Honey" configuration, where columns of hydroxyapatite were inserted within the collagen matrix. The use of different types of scaffolds allowed us to identify the best scaffold in terms of integration and tissue regeneration. The animals included were divided into four groups: three were treated using different types of scaffold while one was left untreated and represented the control group. Evaluations were made at 3 months through CT analysis. The novel "Honey" configuration of the scaffold with hydroxyapatite seems to allow for a better reparative process, although we are still far from obtaining a complete restoration of the defect at this time point of follow-up.

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