Evaluation of INNO-LiPA MYCOBACTERIA v2: Improved reverse hybridization multiple DNA probe assay for mycobacterial identification

Enrico Tortoli, Alessandro Mariottini, Gianna Mazzarelli

Research output: Contribution to journalArticle

Abstract

INNO-LiPA MYCOBACTERIA (Innogenetics, Ghent, Belgium) is a reverse hybridization DNA probe assay that has been recently improved by increasing the number of identifiable mycobacterial species to 16. Our assessment, performed with 197 mycobacteria belonging to 81 taxa, revealed 100% specificity and sensitivity for 20 out of 23 probes. The probes specific for Mycobacterium fortuitum complex, for the Mycobacterium avium-intracellulare-scrofulaceum group, and for Mycobacterium intracellulare type 2 cross-reacted with several mycobacteria rarely isolated from clinical specimens. The overall sensitivity was 100%, and the overall specificity was 94.4%.

Original languageEnglish
Pages (from-to)4418-4420
Number of pages3
JournalJournal of Clinical Microbiology
Volume41
Issue number9
DOIs
Publication statusPublished - Sep 1 2003

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Mycobacterium avium Complex
DNA Probes
Mycobacterium
Mycobacterium fortuitum
Belgium
Sensitivity and Specificity

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Evaluation of INNO-LiPA MYCOBACTERIA v2 : Improved reverse hybridization multiple DNA probe assay for mycobacterial identification. / Tortoli, Enrico; Mariottini, Alessandro; Mazzarelli, Gianna.

In: Journal of Clinical Microbiology, Vol. 41, No. 9, 01.09.2003, p. 4418-4420.

Research output: Contribution to journalArticle

Tortoli, Enrico ; Mariottini, Alessandro ; Mazzarelli, Gianna. / Evaluation of INNO-LiPA MYCOBACTERIA v2 : Improved reverse hybridization multiple DNA probe assay for mycobacterial identification. In: Journal of Clinical Microbiology. 2003 ; Vol. 41, No. 9. pp. 4418-4420.
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