Evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for identification and clustering of Neisseria gonorrhoeae Clinical microbiology and vaccines

Anna Carannante, Elena De Carolis, Paola Vacca, Antonietta Vella, Caterina Vocale, Maria Antonia De Francesco, Marco Cusini, Simonetta Del Re, Ivano Dal Conte, Antonio Cristaudo, Patrizia Ober, Maurizio Sanguinetti, Paola Stefanelli

Research output: Contribution to journalArticle

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Abstract

Background: The sexually transmitted infection gonorrhea remains a public health concern for becoming resistant to drug treatments available. The purpose of this study was to evaluate the usefulness of the matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) to identify and cluster Neisseria gonorrhoeae. From a current monitoring in Italy, as part of the European Gonococcal Antimicrobial Surveillance Programme (EURO-GASP), 93 gonococci collected from 2007 to 2012 susceptible (44 isolates) and resistant (49 isolates) to cefixime were selected. Minimum Inhibitory Concentration (MIC) values for cefixime was assessed by Etest carried out in agreement with the manufacturer's instructions and interpreted referring to European Committee on Antimicrobial Susceptibility testing (EUCAST) clinical breakpoints criteria. Data obtained by N. gonorrhoeae multiantigen sequence typing (NG-MAST) and the dendrogram based on the concatenation of porB and tbpB genes were evaluated. MALDI-TOF MS, to reconfirm gonorrhea identification, analyzed single colonies from freshly grown isolates and applied directly on a ground-steel MALDI target plate. For the MALDI-TOF dendrogram cluster analysis, MSPs (Main Spectrum Profile) from each isolate were created acquiring 5000 shots from 10 technical replicates obtained from bacteria extraction. Results: Molecular typing by NG-MAST showed 28 sequence types (STs); G1407 was the predominant accounting for 75 gonococci. All the 93 gonococci, except one, were correctly identified at species level by MALDI-TOF MS and G1407 isolates were divided into two clusters. Conclusion: MALDI-TOF MS for a real-time detection and cluster analysis of gonorrhea is a promising tool for surveillance purposes. Moreover, additional studies are required to collect more data on the performance of MALDI-TOF MS for gonococci.

Original languageEnglish
Article number142
JournalBMC Microbiology
Volume15
Issue number1
DOIs
Publication statusPublished - Jul 24 2015

Fingerprint

Neisseria gonorrhoeae
Microbiology
Cluster Analysis
Mass Spectrometry
Lasers
Vaccines
Gonorrhea
Cefixime
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Disk Diffusion Antimicrobial Tests
Molecular Typing
Steel
Microbial Sensitivity Tests
Sexually Transmitted Diseases
Italy
Public Health
Bacteria
Pharmaceutical Preparations
Genes

Keywords

  • Cluster
  • G1407
  • MALDI-TOF MS
  • Neisseria gonorrhoeae

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for identification and clustering of Neisseria gonorrhoeae Clinical microbiology and vaccines. / Carannante, Anna; De Carolis, Elena; Vacca, Paola; Vella, Antonietta; Vocale, Caterina; De Francesco, Maria Antonia; Cusini, Marco; Del Re, Simonetta; Dal Conte, Ivano; Cristaudo, Antonio; Ober, Patrizia; Sanguinetti, Maurizio; Stefanelli, Paola.

In: BMC Microbiology, Vol. 15, No. 1, 142, 24.07.2015.

Research output: Contribution to journalArticle

Carannante, Anna ; De Carolis, Elena ; Vacca, Paola ; Vella, Antonietta ; Vocale, Caterina ; De Francesco, Maria Antonia ; Cusini, Marco ; Del Re, Simonetta ; Dal Conte, Ivano ; Cristaudo, Antonio ; Ober, Patrizia ; Sanguinetti, Maurizio ; Stefanelli, Paola. / Evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for identification and clustering of Neisseria gonorrhoeae Clinical microbiology and vaccines. In: BMC Microbiology. 2015 ; Vol. 15, No. 1.
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AU - Vella, Antonietta

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AU - Cusini, Marco

AU - Del Re, Simonetta

AU - Dal Conte, Ivano

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