Evaluation of osteoblast-like cell response to Proroot™ MTA (mineral trioxide aggregate) cement

G. A. Pelliccioni, G. Ciapetti, E. Cenni, D. Granchi, M. Nanni, S. Pagani, A. Giunti

Research output: Contribution to journalArticle


Some endodontic sealers have been shown to cause local and systemic effects, mainly due to microleakage of chemicals from the sealer. To avoid the risk of toxic effects in vivo, the biological compatibility of filling materials has to be assessed. In vitro compatibility of Proroot™ MTA cement in comparison with two different fillers used in clinical practice, was examined by testing the adherence, viability, proliferation and secretion of collagen of osteoblast-like cells. In our experimental system, Saos-2 cells challenged with Proroot™ MTA for 24 and 72 h showed a better behaviour than the cells exposed to the other compounds under assay. We found that the cells attached to the rough surface of Proroot™ MTA cement and spread onto the rough surface. Moreover, the cells on Proroot™ MTA were viable, grew, and released some collagen even at 72 h, while cell metabolism and growth was dramatically reduced onto sEBA and amalgam surfaces. A parallel behaviour was found after the cells were challenged with extracts of the different fillers. In conclusion, according to our in vitro study, Proroot™ MTA showed a good interaction with bone-forming cells: such behaviour may partially account for its satisfying clinical performance.

Original languageEnglish
Pages (from-to)167-173
Number of pages7
JournalJournal of Materials Science: Materials in Medicine
Issue number2
Publication statusPublished - Feb 2004


ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Chemical Engineering(all)

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