TY - JOUR
T1 - Evaluation of osteoconduction of a synthetic hydroxyapatite/β-tricalcium phosphate block fixed in rabbit mandibles
AU - Pires, Luis Carlos de Almeida
AU - da Silva, Rodrigo Capalbo
AU - Poli, Pier Paolo
AU - Esgalha, Fernando Ruas
AU - Hadad, Henrique
AU - Palin, Letícia Pitol
AU - Santos, Ana Flávia Piquera
AU - Colombo, Luara Teixiera
AU - de Jesus, Laís Kawamata
AU - Bassi, Ana Paula Farnezi
AU - Maiorana, Carlo
AU - Okamoto, Roberta
AU - de Carvalho, Paulo Sérgio Perri
AU - Souza, Francisley Ávila
N1 - Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/11/1
Y1 - 2020/11/1
N2 - (1) Background: This study aimed to evaluate the incorporation of hydroxyapatite/β-tricalcium phosphate blocks grafted in rabbit mandibles. (2) Methods: Topographic characterization of biomaterial was performed through scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy (SEM-EDX). Ten rabbits randomly received autogenous bone graft harvested from the tibia (Autogenous Group—AG) or synthetic biomaterial manufactured in β-tricalcium phosphate (Biomaterial Group—BG) at their right and left mandibular angles. Euthanasia was performed at 30 and 60 postoperative days; (3) Results: SEM-EDX showed a surface with the formation of crystals clusters. Histological analyses in BG at 30 days showed a slower process of incorporation than AG. At 60 days, BG showed remnants of biomaterial enveloped by bone tissue in the anabolic modeling phase. Histometric analysis showed that mean values of newly formed bone-like tissue in the AG (6.56%/9.70%) were statistically higher compared to BG (3.14%/6.43%) in both periods, respectively. Immunohistochemical analysis demonstrated early bone formation and maturation in the AG with more intense osteopontin and osteocalcin staining. (4) Conclusions: The biomaterial proved to be a possible bone substitute, being incorporated into the receiving bed; however, it showed delayed bone incorporation compared to autogenous bone.
AB - (1) Background: This study aimed to evaluate the incorporation of hydroxyapatite/β-tricalcium phosphate blocks grafted in rabbit mandibles. (2) Methods: Topographic characterization of biomaterial was performed through scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy (SEM-EDX). Ten rabbits randomly received autogenous bone graft harvested from the tibia (Autogenous Group—AG) or synthetic biomaterial manufactured in β-tricalcium phosphate (Biomaterial Group—BG) at their right and left mandibular angles. Euthanasia was performed at 30 and 60 postoperative days; (3) Results: SEM-EDX showed a surface with the formation of crystals clusters. Histological analyses in BG at 30 days showed a slower process of incorporation than AG. At 60 days, BG showed remnants of biomaterial enveloped by bone tissue in the anabolic modeling phase. Histometric analysis showed that mean values of newly formed bone-like tissue in the AG (6.56%/9.70%) were statistically higher compared to BG (3.14%/6.43%) in both periods, respectively. Immunohistochemical analysis demonstrated early bone formation and maturation in the AG with more intense osteopontin and osteocalcin staining. (4) Conclusions: The biomaterial proved to be a possible bone substitute, being incorporated into the receiving bed; however, it showed delayed bone incorporation compared to autogenous bone.
KW - Biocompatibility
KW - Biomaterials
KW - Bone augmentation
KW - Bone conduction
KW - Bone grafting
KW - Calcium hydroxyapatite
KW - Tissue regeneration
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U2 - 10.3390/ma13214902
DO - 10.3390/ma13214902
M3 - Article
AN - SCOPUS:85094864275
VL - 13
SP - 1
EP - 16
JO - Materials
JF - Materials
SN - 1996-1944
IS - 21
M1 - 4902
ER -