Evaluation of osteoconduction of biphasic calcium phosphate ceramic in the calvaria of rats: Microscopic and histometric analysis

Igor De Oliveira Puttini, Pier Paolo Poli, Carlo Maiorana, Igor Rodrigues De Vasconcelos, Luis Eduardo Schmidt, Luara Teixeira Colombo, Henrique Hadad, Gabriel Mulinari Dos Santos, Paulo Sergio Perri De Carvalho, Francisley Ávila Souza

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(1) Background: Evaluate the osteoconduction capability of a biphasic calcium phosphate (BCP) ceramic composed of hydroxyapatite and β-tricalcium phosphate 60%/40% in a rat model. (2) Methods: In the calvarial bone of 54 adult male rats, 7-mm diameter critical size defects were performed. The animals were randomly allocated to three experimental groups according to the type of material: blood clot (BCG), blood clot covered with a bovine-derived collagen membrane (MBCG), and BCP ceramic covered with a bovine-derived collagen membrane (BCPG). In each group, 6 animals were euthanatized at post-operative days 7, 30, and 60 for histological and histometric analysis. (3) Results: The qualitative analysis revealed the persistence of the collagen membrane at seven days, with no relevant newly bone formation in all groups. At 30 days, centripetal bone formation was observed residual particles of the biomaterial surrounded by fibroblasts noted in the BCPG. At 60 days, while BCG and MBCG showed a partial maturation with the central part of the defect populated by a fibrous connective tissue, in the BCPG the critical area was entirely occupied by newly formed bone. In the intra groups analysis was noted a significant increase in new bone formation during the experimental period (p < 0.05). At 60 days, BCPG showed a higher percentage area of new bone formation (p < 0.05). (4) Conclusion: BCP promoted a new bone formation by osteoconduction and might be considered a valid alternative in bone regeneration procedures.

Original languageEnglish
Article numberE7
JournalJournal of Functional Biomaterials
Issue number1
Publication statusPublished - Jan 1 2019


  • Biphasic calcium phosphate
  • Bone regeneration
  • Bone substitute
  • Ceramics

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering


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