Abstract
(1) Background: Evaluate the osteoconduction capability of a biphasic calcium phosphate (BCP) ceramic composed of hydroxyapatite and β-tricalcium phosphate 60%/40% in a rat model. (2) Methods: In the calvarial bone of 54 adult male rats, 7-mm diameter critical size defects were performed. The animals were randomly allocated to three experimental groups according to the type of material: blood clot (BCG), blood clot covered with a bovine-derived collagen membrane (MBCG), and BCP ceramic covered with a bovine-derived collagen membrane (BCPG). In each group, 6 animals were euthanatized at post-operative days 7, 30, and 60 for histological and histometric analysis. (3) Results: The qualitative analysis revealed the persistence of the collagen membrane at seven days, with no relevant newly bone formation in all groups. At 30 days, centripetal bone formation was observed residual particles of the biomaterial surrounded by fibroblasts noted in the BCPG. At 60 days, while BCG and MBCG showed a partial maturation with the central part of the defect populated by a fibrous connective tissue, in the BCPG the critical area was entirely occupied by newly formed bone. In the intra groups analysis was noted a significant increase in new bone formation during the experimental period (p < 0.05). At 60 days, BCPG showed a higher percentage area of new bone formation (p < 0.05). (4) Conclusion: BCP promoted a new bone formation by osteoconduction and might be considered a valid alternative in bone regeneration procedures.
| Original language | English |
|---|---|
| Article number | E7 |
| Journal | Journal of Functional Biomaterials |
| Volume | 10 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 1 2019 |
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Keywords
- Biphasic calcium phosphate
- Bone regeneration
- Bone substitute
- Ceramics
ASJC Scopus subject areas
- Biomaterials
- Biomedical Engineering
Cite this
Evaluation of osteoconduction of biphasic calcium phosphate ceramic in the calvaria of rats : Microscopic and histometric analysis. / De Oliveira Puttini, Igor; Poli, Pier Paolo; Maiorana, Carlo; De Vasconcelos, Igor Rodrigues; Schmidt, Luis Eduardo; Colombo, Luara Teixeira; Hadad, Henrique; Dos Santos, Gabriel Mulinari; De Carvalho, Paulo Sergio Perri; Souza, Francisley Ávila.
In: Journal of Functional Biomaterials, Vol. 10, No. 1, E7, 01.01.2019.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Evaluation of osteoconduction of biphasic calcium phosphate ceramic in the calvaria of rats
T2 - Microscopic and histometric analysis
AU - De Oliveira Puttini, Igor
AU - Poli, Pier Paolo
AU - Maiorana, Carlo
AU - De Vasconcelos, Igor Rodrigues
AU - Schmidt, Luis Eduardo
AU - Colombo, Luara Teixeira
AU - Hadad, Henrique
AU - Dos Santos, Gabriel Mulinari
AU - De Carvalho, Paulo Sergio Perri
AU - Souza, Francisley Ávila
PY - 2019/1/1
Y1 - 2019/1/1
N2 - (1) Background: Evaluate the osteoconduction capability of a biphasic calcium phosphate (BCP) ceramic composed of hydroxyapatite and β-tricalcium phosphate 60%/40% in a rat model. (2) Methods: In the calvarial bone of 54 adult male rats, 7-mm diameter critical size defects were performed. The animals were randomly allocated to three experimental groups according to the type of material: blood clot (BCG), blood clot covered with a bovine-derived collagen membrane (MBCG), and BCP ceramic covered with a bovine-derived collagen membrane (BCPG). In each group, 6 animals were euthanatized at post-operative days 7, 30, and 60 for histological and histometric analysis. (3) Results: The qualitative analysis revealed the persistence of the collagen membrane at seven days, with no relevant newly bone formation in all groups. At 30 days, centripetal bone formation was observed residual particles of the biomaterial surrounded by fibroblasts noted in the BCPG. At 60 days, while BCG and MBCG showed a partial maturation with the central part of the defect populated by a fibrous connective tissue, in the BCPG the critical area was entirely occupied by newly formed bone. In the intra groups analysis was noted a significant increase in new bone formation during the experimental period (p < 0.05). At 60 days, BCPG showed a higher percentage area of new bone formation (p < 0.05). (4) Conclusion: BCP promoted a new bone formation by osteoconduction and might be considered a valid alternative in bone regeneration procedures.
AB - (1) Background: Evaluate the osteoconduction capability of a biphasic calcium phosphate (BCP) ceramic composed of hydroxyapatite and β-tricalcium phosphate 60%/40% in a rat model. (2) Methods: In the calvarial bone of 54 adult male rats, 7-mm diameter critical size defects were performed. The animals were randomly allocated to three experimental groups according to the type of material: blood clot (BCG), blood clot covered with a bovine-derived collagen membrane (MBCG), and BCP ceramic covered with a bovine-derived collagen membrane (BCPG). In each group, 6 animals were euthanatized at post-operative days 7, 30, and 60 for histological and histometric analysis. (3) Results: The qualitative analysis revealed the persistence of the collagen membrane at seven days, with no relevant newly bone formation in all groups. At 30 days, centripetal bone formation was observed residual particles of the biomaterial surrounded by fibroblasts noted in the BCPG. At 60 days, while BCG and MBCG showed a partial maturation with the central part of the defect populated by a fibrous connective tissue, in the BCPG the critical area was entirely occupied by newly formed bone. In the intra groups analysis was noted a significant increase in new bone formation during the experimental period (p < 0.05). At 60 days, BCPG showed a higher percentage area of new bone formation (p < 0.05). (4) Conclusion: BCP promoted a new bone formation by osteoconduction and might be considered a valid alternative in bone regeneration procedures.
KW - Biphasic calcium phosphate
KW - Bone regeneration
KW - Bone substitute
KW - Ceramics
UR - http://www.scopus.com/inward/record.url?scp=85067098449&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85067098449&partnerID=8YFLogxK
U2 - 10.3390/jfb10010007
DO - 10.3390/jfb10010007
M3 - Article
VL - 10
JO - Journal of Functional Biomaterials
JF - Journal of Functional Biomaterials
SN - 2079-4983
IS - 1
M1 - E7
ER -