Evaluation of p16 and Id1 status and endogenous reference genes in human chondrosarcoma by real-time PCR

Julia Asp, Camilla Brantsing, Karina Lövstedt, Maria Serena Benassi, Sven Inerot, Gabriella Gamberi, Piero Picci, Anders Lindahl

Research output: Contribution to journalArticle

Abstract

Both the tumour suppressor, p16, and the helixloop-helix transcription factor, Id1, have been assigned roles in tumour growth in general and appear to be involved in chondrosarcoma. Id1 has further been found to repress the expression of p16. Therefore, the mRNA expression of these two genes was studied by real-time PCR in a search for prognostic markers in human chondrosarcoma. To get reliable quantitative data, however, the choice of endogenous reference gene for use in the assay is important. Therefore, eleven different endogenous reference genes were evaluated in chondrosarcoma cells and articular chondrocytes. 18S rRNA appeared to be the best choice to use as endogenous reference gene, since it was suitable for both kinds of cells. Several of the other reference genes tested showed variation between individuals or between normal chondrocytes and chondrosarcoma cells. This demonstrates the importance of using a correct endogenous reference gene to get reliable results from quantitative measurements. Both p16 and Id1 showed varied gene expression patterns among the samples and none of these genes could be significantly correlated to prognosis.

Original languageEnglish
Pages (from-to)1577-1582
Number of pages6
JournalInternational Journal of Oncology
Volume27
Issue number6
Publication statusPublished - Dec 2005

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Keywords

  • Articular chondrocytes
  • Chondrosarcoma
  • Endogenous reference genes
  • Id1
  • P16

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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