TY - JOUR
T1 - Evaluation of p16 and Id1 status and endogenous reference genes in human chondrosarcoma by real-time PCR
AU - Asp, Julia
AU - Brantsing, Camilla
AU - Lövstedt, Karina
AU - Benassi, Maria Serena
AU - Inerot, Sven
AU - Gamberi, Gabriella
AU - Picci, Piero
AU - Lindahl, Anders
PY - 2005/12
Y1 - 2005/12
N2 - Both the tumour suppressor, p16, and the helixloop-helix transcription factor, Id1, have been assigned roles in tumour growth in general and appear to be involved in chondrosarcoma. Id1 has further been found to repress the expression of p16. Therefore, the mRNA expression of these two genes was studied by real-time PCR in a search for prognostic markers in human chondrosarcoma. To get reliable quantitative data, however, the choice of endogenous reference gene for use in the assay is important. Therefore, eleven different endogenous reference genes were evaluated in chondrosarcoma cells and articular chondrocytes. 18S rRNA appeared to be the best choice to use as endogenous reference gene, since it was suitable for both kinds of cells. Several of the other reference genes tested showed variation between individuals or between normal chondrocytes and chondrosarcoma cells. This demonstrates the importance of using a correct endogenous reference gene to get reliable results from quantitative measurements. Both p16 and Id1 showed varied gene expression patterns among the samples and none of these genes could be significantly correlated to prognosis.
AB - Both the tumour suppressor, p16, and the helixloop-helix transcription factor, Id1, have been assigned roles in tumour growth in general and appear to be involved in chondrosarcoma. Id1 has further been found to repress the expression of p16. Therefore, the mRNA expression of these two genes was studied by real-time PCR in a search for prognostic markers in human chondrosarcoma. To get reliable quantitative data, however, the choice of endogenous reference gene for use in the assay is important. Therefore, eleven different endogenous reference genes were evaluated in chondrosarcoma cells and articular chondrocytes. 18S rRNA appeared to be the best choice to use as endogenous reference gene, since it was suitable for both kinds of cells. Several of the other reference genes tested showed variation between individuals or between normal chondrocytes and chondrosarcoma cells. This demonstrates the importance of using a correct endogenous reference gene to get reliable results from quantitative measurements. Both p16 and Id1 showed varied gene expression patterns among the samples and none of these genes could be significantly correlated to prognosis.
KW - Articular chondrocytes
KW - Chondrosarcoma
KW - Endogenous reference genes
KW - Id1
KW - P16
UR - http://www.scopus.com/inward/record.url?scp=33644826578&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33644826578&partnerID=8YFLogxK
M3 - Article
C2 - 16273214
AN - SCOPUS:33644826578
VL - 27
SP - 1577
EP - 1582
JO - International Journal of Oncology
JF - International Journal of Oncology
SN - 1019-6439
IS - 6
ER -