Evaluation of platelet concentrates prepared from buffy coats and stored in a glucose-free crystalloid medium

F. Bertolini, P. Rebulla, D. Riccardi, M. Cortellaro, M. L. Ranzi, G. Sirchia

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Abstract

Comparison was made between platelet concentrates prepared from pools of buffy coats removed from standard blood donations and stored in a glucose-free, commercially available crystalloid solution (BC-PCs) and standard platelet concentrates prepared from platelet-rich plasma (PRP-PCs). Platelet yield in BC-PCs and PRP-PCs was 59 and 75 percent of donated platelets, respectively. The number of total white cells in 1 BC-PC unit, prepared from a pool of 7 buffy coats, was 21 x 106, i.e., 50 times lower than that of 7 units of PRP-PCs. The in vitro values of adequate platelet quality were maintained for 10 days in BC-PCs stored in 1000-mL polyolefin bags. Prolonged bleeding times were reduced or corrected in three of three thrombocytopenic leukemic patients evaluated before and after transfusion of stored BC-PCs. Pretransfusion and 1- and 24-hour posttransfusion median platelet counts in 57 leukemic recipients during 4 months of routine transfusion of BC-PCs (n = 93) were 14, 35, and 27 x 109 per L, while those of PRP-PCs (n = 246) were 13, 37, and 31 x 109 per L, respectively. No reactions to BC-PCs were reported, but a 1.3 percent rate of reaction to PRP-PC transfusions was reported. This study indicates that BC-PCs are a good alternative to PRP-PCs for platelet support of thrombocytopenic patients.

Original languageEnglish
Pages (from-to)605-609
Number of pages5
JournalTransfusion
Volume29
Issue number7
Publication statusPublished - 1989

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ASJC Scopus subject areas

  • Immunology
  • Hematology

Cite this

Bertolini, F., Rebulla, P., Riccardi, D., Cortellaro, M., Ranzi, M. L., & Sirchia, G. (1989). Evaluation of platelet concentrates prepared from buffy coats and stored in a glucose-free crystalloid medium. Transfusion, 29(7), 605-609.