Evaluation of platelet function with the PFA-100 system in patients with congenital defects of platelet secretion

Marco Cattaneo, Anna Lecchi, Barbara Agati, Rossana Lombardi, Maddalena L. Zighetti

Research output: Contribution to journalArticlepeer-review

Abstract

The template bleeding time is still the screening test for defects of platelet function, although it is an invasive and poorly reproducible technique. The PFA-100 measures platelet function at high shear. Whole blood is aspirated through a capillary to an aperture of a membrane coated with platelet agonists. The system measures the time required to obtain occlusion of the aperture by a platelet plug (closure time). We measured the closure times in the PFA-100 system and the bleeding time in seven patients with δ-storage pool deficiency, 10 patients with 'primary secretion defect' (not due to abnormalities of platelet granules or the arachidonate pathway), and 40 controls. Measurements were repeated 1 and 4 hours after intravenous infusion of desmopressin in six δ-storage pool deficiency and eight primary secretion defect patients. Baseline bleeding time and closure times with the collagen/epinephrine cartridge were longer in δ-storage pool deficiency and primary secretion defect patients than in controls. In contrast, closure times with the collagen/adenosine diphosphate cartridge were normal in both δ-storage pool deficiency and primary secretion defect patients. Treatment with desmopressin increased the plasma von Willebrand Factor levels, shortened the prolonged bleeding time, shortened the closure times with the collagen/adenosine diphosphate cartridge, and normalized the closure times with the collagen/epinephrine cartridge. Therefore, the PFA-100 test may be a less invasive alternative to the bleeding time in the diagnosis and therapeutic monitoring of patients with platelet secretion defects. The collagen/epinephrine cartridge is more sensitive than the collagen/adenosine diphosphate cartridge to defects of platelet secretion. Copyright (C) 1999 Elsevier Science Ltd.

Original languageEnglish
Pages (from-to)213-217
Number of pages5
JournalThrombosis Research
Volume96
Issue number3
DOIs
Publication statusPublished - Nov 1 1999

Keywords

  • Bleeding time
  • Desmopressin
  • Platelet aggregation
  • Platelet secretion
  • Shear stress

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Hematology

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