Evaluation of Real Time PCR Aspergillus spp. in bronchoalveolar lavage samples

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Abstract

The present study investigated the improvement in the diagnosis of invasive pulmonary aspergillosis (IPA) adding a molecular test on bronchoalveolar lavage (BAL) to the routine diagnostic approach including microscopy, culture and galactomannan (GM) immunoassay. A total of 133 BAL samples were retrospectively tested for the Aspergillus DNA: 112 samples were from immunocompromised patients at risk of invasive fungal infection and 21 were from patients not at risk and without clinical evidence of IPA. The latter samples were used to identify the cut-off of positivity for the molecular test. Applying the cut-off quantity of 50 copies/reaction, the PCR test had 90% sensitivity and 97% specificity and resulted the most sensitive, specific and accurate among those evaluated. The statistical analysis showed that the probability that a patient is not affected by IPA is 99% when the three tests (PCR, GM and culture) are concordantly negative.

Original languageEnglish
Pages (from-to)67-70
Number of pages4
JournalNew Microbiologica
Volume41
Issue number1
Publication statusPublished - Jan 1 2018

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Invasive Pulmonary Aspergillosis
Bronchoalveolar Lavage
Aspergillus
Real-Time Polymerase Chain Reaction
Polymerase Chain Reaction
Immunocompromised Host
Immunoassay
Microscopy
Sensitivity and Specificity
DNA
galactomannan

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

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abstract = "The present study investigated the improvement in the diagnosis of invasive pulmonary aspergillosis (IPA) adding a molecular test on bronchoalveolar lavage (BAL) to the routine diagnostic approach including microscopy, culture and galactomannan (GM) immunoassay. A total of 133 BAL samples were retrospectively tested for the Aspergillus DNA: 112 samples were from immunocompromised patients at risk of invasive fungal infection and 21 were from patients not at risk and without clinical evidence of IPA. The latter samples were used to identify the cut-off of positivity for the molecular test. Applying the cut-off quantity of 50 copies/reaction, the PCR test had 90{\%} sensitivity and 97{\%} specificity and resulted the most sensitive, specific and accurate among those evaluated. The statistical analysis showed that the probability that a patient is not affected by IPA is 99{\%} when the three tests (PCR, GM and culture) are concordantly negative.",
author = "Anna Grancini and Anna Orlandi and Giovanna Lunghi and Dario Consonni and Cristina Pozzi and Valeria Rossetti and Alessandro Palleschi and Nicola Fracchiolla and Ernesto Melada and Monica Savioli and Milena Arghittu and Rita Maiavacca and Anna Prigitano",
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T1 - Evaluation of Real Time PCR Aspergillus spp. in bronchoalveolar lavage samples

AU - Grancini, Anna

AU - Orlandi, Anna

AU - Lunghi, Giovanna

AU - Consonni, Dario

AU - Pozzi, Cristina

AU - Rossetti, Valeria

AU - Palleschi, Alessandro

AU - Fracchiolla, Nicola

AU - Melada, Ernesto

AU - Savioli, Monica

AU - Arghittu, Milena

AU - Maiavacca, Rita

AU - Prigitano, Anna

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AB - The present study investigated the improvement in the diagnosis of invasive pulmonary aspergillosis (IPA) adding a molecular test on bronchoalveolar lavage (BAL) to the routine diagnostic approach including microscopy, culture and galactomannan (GM) immunoassay. A total of 133 BAL samples were retrospectively tested for the Aspergillus DNA: 112 samples were from immunocompromised patients at risk of invasive fungal infection and 21 were from patients not at risk and without clinical evidence of IPA. The latter samples were used to identify the cut-off of positivity for the molecular test. Applying the cut-off quantity of 50 copies/reaction, the PCR test had 90% sensitivity and 97% specificity and resulted the most sensitive, specific and accurate among those evaluated. The statistical analysis showed that the probability that a patient is not affected by IPA is 99% when the three tests (PCR, GM and culture) are concordantly negative.

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