Evaluation of reformulated chemiluminescent DNA probe (AccuProbe) for culture identification of Mycobacterium kansasii

Enrico Tortoli; M. Tullia Simonetti; Federica Lavinia

Evaluation of reformulated chemiluminescent DNA probe (AccuProbe) for culture identification of Mycobacterium kansasii

Enrico Tortoli, M. Tullia Simonetti, Federica Lavinia

IRCCS Ospedale San Raffaele

Research output: Contribution to journal › Article › peer-review

Abstract

A panel of 104 isolates belonging to the species Mycobacterium kansasii and 78 mycobacterial isolates belonging to other species was tested in parallel with the present commercially available DNA probe (Accu-Probe; Gen- Probe) and with a new probe just developed by the same manufacturer. While the old version of the probe confirmed the previously reported low sensitivity (only 59% of the M. kansaii isolates reacted), the new one was 100% sensitive. Only two non-M. kansasii strains, both M. gastri isolates, gave false-positive hybridization results.

Original language	English
Pages (from-to)	2838-2840
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	34
Issue number	11
Publication status	Published - Nov 1996

ASJC Scopus subject areas

Microbiology (medical)
Microbiology

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title = "Evaluation of reformulated chemiluminescent DNA probe (AccuProbe) for culture identification of Mycobacterium kansasii",

abstract = "A panel of 104 isolates belonging to the species Mycobacterium kansasii and 78 mycobacterial isolates belonging to other species was tested in parallel with the present commercially available DNA probe (Accu-Probe; Gen- Probe) and with a new probe just developed by the same manufacturer. While the old version of the probe confirmed the previously reported low sensitivity (only 59% of the M. kansaii isolates reacted), the new one was 100% sensitive. Only two non-M. kansasii strains, both M. gastri isolates, gave false-positive hybridization results.",

author = "Enrico Tortoli and Simonetti, {M. Tullia} and Federica Lavinia",

year = "1996",

month = nov,

language = "English",

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journal = "Journal of Clinical Microbiology",

issn = "0095-1137",

publisher = "American Society for Microbiology",

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PY - 1996/11

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AB - A panel of 104 isolates belonging to the species Mycobacterium kansasii and 78 mycobacterial isolates belonging to other species was tested in parallel with the present commercially available DNA probe (Accu-Probe; Gen- Probe) and with a new probe just developed by the same manufacturer. While the old version of the probe confirmed the previously reported low sensitivity (only 59% of the M. kansaii isolates reacted), the new one was 100% sensitive. Only two non-M. kansasii strains, both M. gastri isolates, gave false-positive hybridization results.

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