Evaluation of T3B fingerprinting for identification of clinical and environmental Sporothrix species

Manoel M arques Evangelista Oliveira, Ricardo Franco-Duarte, Orazio Romeo, Célia Pais, Giuseppe Criseo, Paula Sampaio, Rosely M aria Zancope-Oliveira

Research output: Contribution to journalArticle

Abstract

In this study, PCR fingerprinting using the universal primer T3B was applied to distinguish among clinical and environmental species of the Sporothrix complex, Sporothrix brasiliensis, S. globosa, S. mexicana, S. pallida, S. luriei and S. schenckii sensu stricto. The T3B fingerprinting generated clearly distinct banding patterns, allowing the correct identification of all 43 clinical and environmental isolates at the species level, what was confirmed by partial calmodulin gene sequence analyses. This technique is reproducible and provides the identification of all species of the Sporothrix complex with sufficient accuracy to be applied in clinical mycology laboratories as well as in epidemiological studies in order to obtain a better understanding of the epidemiology of sporotrichosis.

Original languageEnglish
JournalFEMS Microbiology Letters
Volume362
Issue number6
DOIs
Publication statusPublished - Mar 1 2015

Keywords

  • molecular identification
  • Sporothrix species complex
  • sporotrichosis

ASJC Scopus subject areas

  • Medicine(all)

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    Oliveira, M. M. A. E., Franco-Duarte, R., Romeo, O., Pais, C., Criseo, G., Sampaio, P., & Zancope-Oliveira, R. M. A. (2015). Evaluation of T3B fingerprinting for identification of clinical and environmental Sporothrix species. FEMS Microbiology Letters, 362(6). https://doi.org/10.1093/femsle/fnv027