Evaluation of the abnormal platelet function in von Willebrand disease by the blood filtration test

Francesco I. Pareti, Marco Cattaneo, Luca Carpinelli, Maddalena L. Zighetti, Caterina Bressi, Pier Mannuccio Mannucci, Zaverio M. Ruggeri

Research output: Contribution to journalArticle

Abstract

We have evaluated platelet function in different subtypes of von Willebrand disease (vWD) by pushing blood through the capillary-sized channels of a glass filter. Patients, including those with type IIB vWD, showed lower than normal platelet retention and increased cumulative number of blood drops passing through the filter as a function of time. In contrast, shear-induced platelet aggregation, measured in the cone-and-plate viscometer, was paradoxically increased in type IIB patients. Treatment with 1-desamino-8-D-arginine vasopressin (DDAVP) tended to normalize the filter test in patients with type I-platelet normal and type I-platelet low vWD, but infusion of a factor VIII/von Willebrand factor (vWF) concentrate lacking the largest vWF multimers was without effect in type 3 patients. Experiments with specific monoclonal antibodies demonstrated that the A1 and A3 domains of vWF, as well as the glycoproteins Ibα and IIb-IIIa on platelets, are required for platelet retention in the filter. Thus, the test may reflect vWF function with regard to both platelet adhesion and aggregation under high shear stress, and provide relevant information on mechanisms involved in primary hemostasis.

Original languageEnglish
Pages (from-to)460-468
Number of pages9
JournalThrombosis and Haemostasis
Volume76
Issue number3
Publication statusPublished - 1996

ASJC Scopus subject areas

  • Hematology

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    Pareti, F. I., Cattaneo, M., Carpinelli, L., Zighetti, M. L., Bressi, C., Mannucci, P. M., & Ruggeri, Z. M. (1996). Evaluation of the abnormal platelet function in von Willebrand disease by the blood filtration test. Thrombosis and Haemostasis, 76(3), 460-468.