Evaluation of the clinical relevance of the anionic glutathione-s-transferase (GSTπ and multidrug resistance (mdr-1) gene coexpression in leukemias and lymphomas

D. Russo, J. P. Marie, D. C. Zhou, A. M. Faussat, C. Melli, D. Damiani, A. Michelutti, M. Michieli, R. Fanin, M. Baccarani, R. Zittoun

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

By using RNA slot-blot technique, the frequency and the degree of GSTπ and mdr-1 gene coexpression were investigated in 23 AML patients, 9 ALL, 9 CLL and 11 cases of NHL in an attempt to study their clinical and prognostic relevance. GSTπ and mdr-1 levels were expressed as arbitrary units (U) with respect to the negative controls (U = 0), MCF7 and HL60 sensitive cell lines, and the positive controls (U = 10), MCF7/DOXO and HL60/DNR resistant cell lines. The concomitant GSTπmdr1 gene overexpression showed a negative prognostic value in the set of newly diagnosed AML pts (10 cases), furthermore higher GSTπ and mdrl mRNA levels were averagely detected in the relapsed/resistant ALL pts (4 cases), and in CLL (7 cases) and NHL (8 cases) heavily pretreated patients who were unresponsive to chemotherapy and with a disease progression. These preliminary data show that two different mechanisms of drug resistance can be coexpressed at the same time in those leukemias and lymphomas with a clinically unfavourable course.

Original languageEnglish
Pages (from-to)453-468
Number of pages16
JournalLeukemia and Lymphoma
Volume15
Issue number5-6
DOIs
Publication statusPublished - 1994

Fingerprint

Multiple Drug Resistance
Glutathione Transferase
Lymphoma
Leukemia
Cell Line
HL-60 Cells
Drug Resistance
Genes
Disease Progression
RNA
Drug Therapy
Messenger RNA
Clinical Studies

Keywords

  • GSTπ
  • Leukemia
  • Lymphoma
  • Mdr-1

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

Cite this

Evaluation of the clinical relevance of the anionic glutathione-s-transferase (GSTπ and multidrug resistance (mdr-1) gene coexpression in leukemias and lymphomas. / Russo, D.; Marie, J. P.; Zhou, D. C.; Faussat, A. M.; Melli, C.; Damiani, D.; Michelutti, A.; Michieli, M.; Fanin, R.; Baccarani, M.; Zittoun, R.

In: Leukemia and Lymphoma, Vol. 15, No. 5-6, 1994, p. 453-468.

Research output: Contribution to journalArticle

Russo, D, Marie, JP, Zhou, DC, Faussat, AM, Melli, C, Damiani, D, Michelutti, A, Michieli, M, Fanin, R, Baccarani, M & Zittoun, R 1994, 'Evaluation of the clinical relevance of the anionic glutathione-s-transferase (GSTπ and multidrug resistance (mdr-1) gene coexpression in leukemias and lymphomas', Leukemia and Lymphoma, vol. 15, no. 5-6, pp. 453-468. https://doi.org/10.3109/10428199409049749
Russo, D. ; Marie, J. P. ; Zhou, D. C. ; Faussat, A. M. ; Melli, C. ; Damiani, D. ; Michelutti, A. ; Michieli, M. ; Fanin, R. ; Baccarani, M. ; Zittoun, R. / Evaluation of the clinical relevance of the anionic glutathione-s-transferase (GSTπ and multidrug resistance (mdr-1) gene coexpression in leukemias and lymphomas. In: Leukemia and Lymphoma. 1994 ; Vol. 15, No. 5-6. pp. 453-468.
@article{e53130271281414bb528644af5e53fd6,
title = "Evaluation of the clinical relevance of the anionic glutathione-s-transferase (GSTπ and multidrug resistance (mdr-1) gene coexpression in leukemias and lymphomas",
abstract = "By using RNA slot-blot technique, the frequency and the degree of GSTπ and mdr-1 gene coexpression were investigated in 23 AML patients, 9 ALL, 9 CLL and 11 cases of NHL in an attempt to study their clinical and prognostic relevance. GSTπ and mdr-1 levels were expressed as arbitrary units (U) with respect to the negative controls (U = 0), MCF7 and HL60 sensitive cell lines, and the positive controls (U = 10), MCF7/DOXO and HL60/DNR resistant cell lines. The concomitant GSTπmdr1 gene overexpression showed a negative prognostic value in the set of newly diagnosed AML pts (10 cases), furthermore higher GSTπ and mdrl mRNA levels were averagely detected in the relapsed/resistant ALL pts (4 cases), and in CLL (7 cases) and NHL (8 cases) heavily pretreated patients who were unresponsive to chemotherapy and with a disease progression. These preliminary data show that two different mechanisms of drug resistance can be coexpressed at the same time in those leukemias and lymphomas with a clinically unfavourable course.",
keywords = "GSTπ, Leukemia, Lymphoma, Mdr-1",
author = "D. Russo and Marie, {J. P.} and Zhou, {D. C.} and Faussat, {A. M.} and C. Melli and D. Damiani and A. Michelutti and M. Michieli and R. Fanin and M. Baccarani and R. Zittoun",
year = "1994",
doi = "10.3109/10428199409049749",
language = "English",
volume = "15",
pages = "453--468",
journal = "Leukemia and Lymphoma",
issn = "1042-8194",
publisher = "Taylor and Francis Ltd.",
number = "5-6",

}

TY - JOUR

T1 - Evaluation of the clinical relevance of the anionic glutathione-s-transferase (GSTπ and multidrug resistance (mdr-1) gene coexpression in leukemias and lymphomas

AU - Russo, D.

AU - Marie, J. P.

AU - Zhou, D. C.

AU - Faussat, A. M.

AU - Melli, C.

AU - Damiani, D.

AU - Michelutti, A.

AU - Michieli, M.

AU - Fanin, R.

AU - Baccarani, M.

AU - Zittoun, R.

PY - 1994

Y1 - 1994

N2 - By using RNA slot-blot technique, the frequency and the degree of GSTπ and mdr-1 gene coexpression were investigated in 23 AML patients, 9 ALL, 9 CLL and 11 cases of NHL in an attempt to study their clinical and prognostic relevance. GSTπ and mdr-1 levels were expressed as arbitrary units (U) with respect to the negative controls (U = 0), MCF7 and HL60 sensitive cell lines, and the positive controls (U = 10), MCF7/DOXO and HL60/DNR resistant cell lines. The concomitant GSTπmdr1 gene overexpression showed a negative prognostic value in the set of newly diagnosed AML pts (10 cases), furthermore higher GSTπ and mdrl mRNA levels were averagely detected in the relapsed/resistant ALL pts (4 cases), and in CLL (7 cases) and NHL (8 cases) heavily pretreated patients who were unresponsive to chemotherapy and with a disease progression. These preliminary data show that two different mechanisms of drug resistance can be coexpressed at the same time in those leukemias and lymphomas with a clinically unfavourable course.

AB - By using RNA slot-blot technique, the frequency and the degree of GSTπ and mdr-1 gene coexpression were investigated in 23 AML patients, 9 ALL, 9 CLL and 11 cases of NHL in an attempt to study their clinical and prognostic relevance. GSTπ and mdr-1 levels were expressed as arbitrary units (U) with respect to the negative controls (U = 0), MCF7 and HL60 sensitive cell lines, and the positive controls (U = 10), MCF7/DOXO and HL60/DNR resistant cell lines. The concomitant GSTπmdr1 gene overexpression showed a negative prognostic value in the set of newly diagnosed AML pts (10 cases), furthermore higher GSTπ and mdrl mRNA levels were averagely detected in the relapsed/resistant ALL pts (4 cases), and in CLL (7 cases) and NHL (8 cases) heavily pretreated patients who were unresponsive to chemotherapy and with a disease progression. These preliminary data show that two different mechanisms of drug resistance can be coexpressed at the same time in those leukemias and lymphomas with a clinically unfavourable course.

KW - GSTπ

KW - Leukemia

KW - Lymphoma

KW - Mdr-1

UR - http://www.scopus.com/inward/record.url?scp=0028114208&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028114208&partnerID=8YFLogxK

U2 - 10.3109/10428199409049749

DO - 10.3109/10428199409049749

M3 - Article

C2 - 7874003

AN - SCOPUS:0028114208

VL - 15

SP - 453

EP - 468

JO - Leukemia and Lymphoma

JF - Leukemia and Lymphoma

SN - 1042-8194

IS - 5-6

ER -