Evaluation of the immune benefits of two probiotic strains Bifidobacterium animalis ssp. lactis, BB-12 ® and Lactobacillus paracasei ssp. paracasei, L. casei 431 ® in an influenza vaccination model: A randomised, double-blind, placebo-controlled study

Giuliano Rizzardini, Dorte Eskesen, Philip C. Calder, Amedeo Capetti, Lillian Jespersen, Mario Clerici

Research output: Contribution to journalArticlepeer-review

Abstract

The present study investigated the ability of Bifidobacterium animalis ssp. lactis (BB-12 ®) and Lactobacillus paracasei ssp. paracasei (L. casei 431 ®) to modulate the immune system using a vaccination model in healthy subjects. A randomised, double-blind, placebo-controlled, parallel-group study was conducted in 211 subjects (56% females, mean age 33•2 (sd 13•1) years). Subjects consumed a minimum of 10 9 colony-forming units of BB-12 ® (capsule) or L. casei 431 ® (dairy drink) or a matching placebo once daily for 6 weeks. After 2 weeks, a seasonal influenza vaccination was given. Plasma and saliva samples were collected at baseline and after 6 weeks for the analysis of antibodies, cytokines and innate immune parameters. Changes from baseline in vaccine-specific plasma IgG, IgG1 and IgG3 were significantly greater in both probiotic groups v. the corresponding placebo group (L. casei 431 ®, P = 0•01 for IgG; P <0•001 for remaining comparisons). The number of subjects obtaining a substantial increase in specific IgG (defined as ≥ 2-fold above baseline) was significantly greater in both probiotic groups v. placebo (BB-12 ®, P <0•001 for IgG, IgG1 and IgG3; L. casei 431 ®, P <0•001 for IgG1 and IgG3). Significantly greater mean fold increases for vaccine-specific secretory IgA in saliva were observed in both probiotic groups v. placebo (BB-12 ®, P = 0•017; L. casei 431 ®, P = 0•035). Similar results were observed for total antibody concentrations. No differences were found for plasma cytokines or innate immune parameters. Data herein show that supplementation with BB-12 ® or L. casei 431 ® may be an effective means to improve immune function by augmenting systemic and mucosal immune responses to challenge.

Original languageEnglish
Pages (from-to)876-884
Number of pages9
JournalBritish Journal of Nutrition
Volume107
Issue number6
DOIs
Publication statusPublished - Mar 28 2012

Keywords

  • Clinical trials
  • Immune modulation
  • Probiotics
  • Vaccination response

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

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