Evaluation of the new NucliSENS EasyQ KPC test for rapid detection of Klebsiella pneumoniae carbapenemase genes (blaKPC)

Teresa Spanu, Barbara Fiori, Tiziana D'Inzeo, Giulia Canu, Serena Campoli, Tommaso Giani, Ivana Palucci, Mario Tumbarello, Maurizio Sanguinetti, Gian Maria Rossolini

Research output: Contribution to journalArticle

Abstract

KPC-type carbapenemases are emerging in Klebsiella pneumoniae and other Gram-negative pathogens worldwide. Rapid and sensitive detection of these resistance determinants has become relevant to clinical management and infection control. We evaluated the bioMérieux EasyQ real-time PCR assay for blaKPC detection with 300 members of the Enterobacteriaceae, including 29 control strains producing known carbapenemases and 271 nonreplicate clinical isolates. The EasyQ assay correctly detected all of the 111 isolates harboring blaKPC genes, with no false positives, and results were available within 2 h.

Original languageEnglish
Pages (from-to)2783-2785
Number of pages3
JournalJournal of Clinical Microbiology
Volume50
Issue number8
DOIs
Publication statusPublished - Aug 2012

ASJC Scopus subject areas

  • Microbiology (medical)

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    Spanu, T., Fiori, B., D'Inzeo, T., Canu, G., Campoli, S., Giani, T., Palucci, I., Tumbarello, M., Sanguinetti, M., & Rossolini, G. M. (2012). Evaluation of the new NucliSENS EasyQ KPC test for rapid detection of Klebsiella pneumoniae carbapenemase genes (blaKPC). Journal of Clinical Microbiology, 50(8), 2783-2785. https://doi.org/10.1128/JCM.00284-12