Abstract
Fungaemia is an increasing nosocomial pathology. The 'gold standard' for detection of fungaemia is blood culture, but it is time-consuming and its sensitivity for early detection is low. On the other hand, yeasts present different antifungal sensitivity patterns to be quickly detected to allow an effective treatment. The aim of this study was to evaluate the diagnostic performances of PNA-FISH to directly identify yeasts from blood cultures and to compare results with those obtained by culture. A total of 176 blood cultures positive for yeasts at direct Gram stain and 24 negative blood cultures as control collected from 15 Italian hospitals, included in a network coordinated by the Medical Mycology Committee, Italian Society of Clinical Microbiology (AMCLI), were examined both by culture and PNA-FISH technology. Sensitivity of the PNA-FISH technique evaluated for five Candida species was 99.3% and specificity, 100%. Distinguishing which yeast is implicated in fungaemia and whether the infection is caused by multiple species are important for the selection of antifungal therapy. The PNA-FISH technique is a very useful approach because the test discriminates between groups of Candida species with different susceptibility pattern, particularly against azoles and echinocandins, with only a 90-minute turn-around time after the Gram-stain reading.
| Original language | English |
|---|---|
| Pages (from-to) | 388-392 |
| Number of pages | 5 |
| Journal | Mycoses |
| Volume | 55 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - Sep 2012 |
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Keywords
- Blood cultures
- Fluorescence in situ hybridisation
- Identification
- Peptide nucleic acid
- Yeast
ASJC Scopus subject areas
- Dermatology
- Infectious Diseases
Cite this
Evaluation of the peptide nucleic acid fluorescence in situ hybridisation technology for yeast identification directly from positive blood cultures : An Italian experience. / Farina, Claudio; Perin, Silvana; Andreoni, Stefano; Conte, Marco; Fazii, Paolo; Lombardi, Gianluigi; Manso, Esther; Morazzoni, Cristina; Sanna, Silvana; Bonetti, Cristina; Carretto, Edoardo; Casella, Pietro; Luzzaro, Francesco; Marone, Piero; Passera, Marco; Rocchetti, Andrea; Vigano, Egidio Franco.
In: Mycoses, Vol. 55, No. 5, 09.2012, p. 388-392.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Evaluation of the peptide nucleic acid fluorescence in situ hybridisation technology for yeast identification directly from positive blood cultures
T2 - An Italian experience
AU - Farina, Claudio
AU - Perin, Silvana
AU - Andreoni, Stefano
AU - Conte, Marco
AU - Fazii, Paolo
AU - Lombardi, Gianluigi
AU - Manso, Esther
AU - Morazzoni, Cristina
AU - Sanna, Silvana
AU - Bonetti, Cristina
AU - Carretto, Edoardo
AU - Casella, Pietro
AU - Luzzaro, Francesco
AU - Marone, Piero
AU - Passera, Marco
AU - Rocchetti, Andrea
AU - Vigano, Egidio Franco
PY - 2012/9
Y1 - 2012/9
N2 - Fungaemia is an increasing nosocomial pathology. The 'gold standard' for detection of fungaemia is blood culture, but it is time-consuming and its sensitivity for early detection is low. On the other hand, yeasts present different antifungal sensitivity patterns to be quickly detected to allow an effective treatment. The aim of this study was to evaluate the diagnostic performances of PNA-FISH to directly identify yeasts from blood cultures and to compare results with those obtained by culture. A total of 176 blood cultures positive for yeasts at direct Gram stain and 24 negative blood cultures as control collected from 15 Italian hospitals, included in a network coordinated by the Medical Mycology Committee, Italian Society of Clinical Microbiology (AMCLI), were examined both by culture and PNA-FISH technology. Sensitivity of the PNA-FISH technique evaluated for five Candida species was 99.3% and specificity, 100%. Distinguishing which yeast is implicated in fungaemia and whether the infection is caused by multiple species are important for the selection of antifungal therapy. The PNA-FISH technique is a very useful approach because the test discriminates between groups of Candida species with different susceptibility pattern, particularly against azoles and echinocandins, with only a 90-minute turn-around time after the Gram-stain reading.
AB - Fungaemia is an increasing nosocomial pathology. The 'gold standard' for detection of fungaemia is blood culture, but it is time-consuming and its sensitivity for early detection is low. On the other hand, yeasts present different antifungal sensitivity patterns to be quickly detected to allow an effective treatment. The aim of this study was to evaluate the diagnostic performances of PNA-FISH to directly identify yeasts from blood cultures and to compare results with those obtained by culture. A total of 176 blood cultures positive for yeasts at direct Gram stain and 24 negative blood cultures as control collected from 15 Italian hospitals, included in a network coordinated by the Medical Mycology Committee, Italian Society of Clinical Microbiology (AMCLI), were examined both by culture and PNA-FISH technology. Sensitivity of the PNA-FISH technique evaluated for five Candida species was 99.3% and specificity, 100%. Distinguishing which yeast is implicated in fungaemia and whether the infection is caused by multiple species are important for the selection of antifungal therapy. The PNA-FISH technique is a very useful approach because the test discriminates between groups of Candida species with different susceptibility pattern, particularly against azoles and echinocandins, with only a 90-minute turn-around time after the Gram-stain reading.
KW - Blood cultures
KW - Fluorescence in situ hybridisation
KW - Identification
KW - Peptide nucleic acid
KW - Yeast
UR - http://www.scopus.com/inward/record.url?scp=84865284675&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84865284675&partnerID=8YFLogxK
U2 - 10.1111/j.1439-0507.2011.02166.x
DO - 10.1111/j.1439-0507.2011.02166.x
M3 - Article
C2 - 22233292
AN - SCOPUS:84865284675
VL - 55
SP - 388
EP - 392
JO - Mycoses
JF - Mycoses
SN - 0933-7407
IS - 5
ER -