Evaluation of the peptide nucleic acid fluorescence in situ hybridisation technology for yeast identification directly from positive blood cultures: An Italian experience

Claudio Farina, Silvana Perin, Stefano Andreoni, Marco Conte, Paolo Fazii, Gianluigi Lombardi, Esther Manso, Cristina Morazzoni, Silvana Sanna, Cristina Bonetti, Edoardo Carretto, Pietro Casella, Francesco Luzzaro, Piero Marone, Marco Passera, Andrea Rocchetti, Egidio Franco Vigano

Research output: Contribution to journalArticle

Abstract

Fungaemia is an increasing nosocomial pathology. The 'gold standard' for detection of fungaemia is blood culture, but it is time-consuming and its sensitivity for early detection is low. On the other hand, yeasts present different antifungal sensitivity patterns to be quickly detected to allow an effective treatment. The aim of this study was to evaluate the diagnostic performances of PNA-FISH to directly identify yeasts from blood cultures and to compare results with those obtained by culture. A total of 176 blood cultures positive for yeasts at direct Gram stain and 24 negative blood cultures as control collected from 15 Italian hospitals, included in a network coordinated by the Medical Mycology Committee, Italian Society of Clinical Microbiology (AMCLI), were examined both by culture and PNA-FISH technology. Sensitivity of the PNA-FISH technique evaluated for five Candida species was 99.3% and specificity, 100%. Distinguishing which yeast is implicated in fungaemia and whether the infection is caused by multiple species are important for the selection of antifungal therapy. The PNA-FISH technique is a very useful approach because the test discriminates between groups of Candida species with different susceptibility pattern, particularly against azoles and echinocandins, with only a 90-minute turn-around time after the Gram-stain reading.

Original languageEnglish
Pages (from-to)388-392
Number of pages5
JournalMycoses
Volume55
Issue number5
DOIs
Publication statusPublished - Sep 2012

Fingerprint

Peptide Nucleic Acids
Fungemia
Fluorescence In Situ Hybridization
Yeasts
Technology
Candida
Echinocandins
Mycology
Azoles
Microbiology
Reading
Pathology
Blood Culture
Infection
Gram's stain

Keywords

  • Blood cultures
  • Fluorescence in situ hybridisation
  • Identification
  • Peptide nucleic acid
  • Yeast

ASJC Scopus subject areas

  • Dermatology
  • Infectious Diseases

Cite this

Evaluation of the peptide nucleic acid fluorescence in situ hybridisation technology for yeast identification directly from positive blood cultures : An Italian experience. / Farina, Claudio; Perin, Silvana; Andreoni, Stefano; Conte, Marco; Fazii, Paolo; Lombardi, Gianluigi; Manso, Esther; Morazzoni, Cristina; Sanna, Silvana; Bonetti, Cristina; Carretto, Edoardo; Casella, Pietro; Luzzaro, Francesco; Marone, Piero; Passera, Marco; Rocchetti, Andrea; Vigano, Egidio Franco.

In: Mycoses, Vol. 55, No. 5, 09.2012, p. 388-392.

Research output: Contribution to journalArticle

Farina, C, Perin, S, Andreoni, S, Conte, M, Fazii, P, Lombardi, G, Manso, E, Morazzoni, C, Sanna, S, Bonetti, C, Carretto, E, Casella, P, Luzzaro, F, Marone, P, Passera, M, Rocchetti, A & Vigano, EF 2012, 'Evaluation of the peptide nucleic acid fluorescence in situ hybridisation technology for yeast identification directly from positive blood cultures: An Italian experience', Mycoses, vol. 55, no. 5, pp. 388-392. https://doi.org/10.1111/j.1439-0507.2011.02166.x
Farina, Claudio ; Perin, Silvana ; Andreoni, Stefano ; Conte, Marco ; Fazii, Paolo ; Lombardi, Gianluigi ; Manso, Esther ; Morazzoni, Cristina ; Sanna, Silvana ; Bonetti, Cristina ; Carretto, Edoardo ; Casella, Pietro ; Luzzaro, Francesco ; Marone, Piero ; Passera, Marco ; Rocchetti, Andrea ; Vigano, Egidio Franco. / Evaluation of the peptide nucleic acid fluorescence in situ hybridisation technology for yeast identification directly from positive blood cultures : An Italian experience. In: Mycoses. 2012 ; Vol. 55, No. 5. pp. 388-392.
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