Evaluation of the selenotranscriptome expression in two hepatocellular carcinoma cell lines

Stefano Guariniello, Giovanni Di Bernardo, Giovanni Colonna, Marcella Cammarota, Giuseppe Castello, Susan Costantini

Research output: Contribution to journalArticle

Abstract

Hepatocellular carcinoma (HCC) is the most common type of liver cancer and is still one of the most fatal cancers. Hence, it needs to identify always new putative markers to improve its diagnosis and prognosis. Since the selenium is able to fight the oxidative damage which is one of the major origins of cell damage as well as cancer, we have recently focused our attention on selenoprotein family and their involvement in HCC. In the present paper we have carried out a global analysis of the selenotranscriptome expression in HepG2 and Huh7 cells compared to the normal human hepatocytes by reverse transcription-qPCR (RT-qPCR). Our data showed that in both cells there are three downregulated (DIO1, DIO2, and SELO) and ten upregulated (GPX4, GPX7, SELK, SELM, SELN, SELT, SELV, SEP15, SEPW1, and TrxR1) genes. Additionally, interactomic studies were carried out to evaluate the ability of these down-and upregulated genes to interact between them as well as to identify putative HUB nodes representing the centers of correlation able to exercise a direct control over the coordinated genes.

Original languageEnglish
Article number419561
JournalAnalytical Cellular Pathology
Volume2015
DOIs
Publication statusPublished - 2015

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Medicine
  • Pathology and Forensic Medicine
  • Cell Biology

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    Guariniello, S., Di Bernardo, G., Colonna, G., Cammarota, M., Castello, G., & Costantini, S. (2015). Evaluation of the selenotranscriptome expression in two hepatocellular carcinoma cell lines. Analytical Cellular Pathology, 2015, [419561]. https://doi.org/10.1155/2015/419561