Abstract
OBJECTIVE. The purpose of this study was to compare the spectral characteristics of lipids, choline-containing com pounds, and glutamine-glutamate complex assessed with 1H-MR spectroscopy with the histologic findings in patients with chronic hepatitis C. SUBJECTS AND METHODS. Nine healthy controls and 30 patients with biopsy-proven hepatitis C virus-related liver disease participated in this prospective study. Degree of fibrosis and histologic activity were scored according to the METAVIR classification. The percentage of involved hepatocytes was used to grade steatosis. Hepatic spectra were obtained with a 3-T spectroscopic system. Tenfold cross-validated stepwise discriminant analysis was performed to classify disease severity on the basis of the spectroscopic findings. RESULTS. There was a strong correlation between 1H-MR spectroscopically measured lipid concentration and the degree of steatosis at histologic examination (r = 0.9236, p <0.0001). This finding enabled clear separation of groups according to degree of histologically determined steatosis. Variation in lipid concentration was consistent with the degree of steatosis (r = 0.7265, p <0.0001) and stage of fibrosis (r = 0.8156, p <0.0001). In univariate analysis, concentrations of both choline-containing compounds and glutamine-glutamate complex had a direct correlation with histologic grade (p <0.0001) and degree of steatosis (p <0.0001) but not with stage of fibrosis (p > 0.05). In multivariate analysis, the only factor independently associated with concentrations of choline-containing compounds and glutamine-glutamate complex was histologic grade. In cross-validated discriminant analysis based on choline-containing compound, glutamine-glutamate complex, and lipid resonance, 70% (21 of 30) of the histologic grade groups and 73% (22 of 30) of the steatosis groups were correctly classified. CONCLUSION. Hydrogen-1 MR spectroscopy can be an alternative to liver biopsy in the evaluation of steatosis and necroinflammatory activity in liver disease but is not useful for complete evaluation of hepatic fibrosis.
| Original language | English |
|---|---|
| Pages (from-to) | 1331-1339 |
| Number of pages | 9 |
| Journal | American Journal of Roentgenology |
| Volume | 190 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - May 2008 |
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Keywords
- H-MR spectroscopy
- Chronic hepatitis
- Liver fibrosis
- Liver steatosis
ASJC Scopus subject areas
- Radiology Nuclear Medicine and imaging
- Radiological and Ultrasound Technology
Cite this
Evaluation of the severity of chronic hepatitis C with 3-T 1H-MR spectroscopy. / Orlacchio, Antonio; Bolacchi, Francesca; Cadioli, Marcello; Bergamini, Alberto; Cozzolino, Valeria; Angelico, Mario; Simonetti, Giovanni.
In: American Journal of Roentgenology, Vol. 190, No. 5, 05.2008, p. 1331-1339.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Evaluation of the severity of chronic hepatitis C with 3-T 1H-MR spectroscopy
AU - Orlacchio, Antonio
AU - Bolacchi, Francesca
AU - Cadioli, Marcello
AU - Bergamini, Alberto
AU - Cozzolino, Valeria
AU - Angelico, Mario
AU - Simonetti, Giovanni
PY - 2008/5
Y1 - 2008/5
N2 - OBJECTIVE. The purpose of this study was to compare the spectral characteristics of lipids, choline-containing com pounds, and glutamine-glutamate complex assessed with 1H-MR spectroscopy with the histologic findings in patients with chronic hepatitis C. SUBJECTS AND METHODS. Nine healthy controls and 30 patients with biopsy-proven hepatitis C virus-related liver disease participated in this prospective study. Degree of fibrosis and histologic activity were scored according to the METAVIR classification. The percentage of involved hepatocytes was used to grade steatosis. Hepatic spectra were obtained with a 3-T spectroscopic system. Tenfold cross-validated stepwise discriminant analysis was performed to classify disease severity on the basis of the spectroscopic findings. RESULTS. There was a strong correlation between 1H-MR spectroscopically measured lipid concentration and the degree of steatosis at histologic examination (r = 0.9236, p <0.0001). This finding enabled clear separation of groups according to degree of histologically determined steatosis. Variation in lipid concentration was consistent with the degree of steatosis (r = 0.7265, p <0.0001) and stage of fibrosis (r = 0.8156, p <0.0001). In univariate analysis, concentrations of both choline-containing compounds and glutamine-glutamate complex had a direct correlation with histologic grade (p <0.0001) and degree of steatosis (p <0.0001) but not with stage of fibrosis (p > 0.05). In multivariate analysis, the only factor independently associated with concentrations of choline-containing compounds and glutamine-glutamate complex was histologic grade. In cross-validated discriminant analysis based on choline-containing compound, glutamine-glutamate complex, and lipid resonance, 70% (21 of 30) of the histologic grade groups and 73% (22 of 30) of the steatosis groups were correctly classified. CONCLUSION. Hydrogen-1 MR spectroscopy can be an alternative to liver biopsy in the evaluation of steatosis and necroinflammatory activity in liver disease but is not useful for complete evaluation of hepatic fibrosis.
AB - OBJECTIVE. The purpose of this study was to compare the spectral characteristics of lipids, choline-containing com pounds, and glutamine-glutamate complex assessed with 1H-MR spectroscopy with the histologic findings in patients with chronic hepatitis C. SUBJECTS AND METHODS. Nine healthy controls and 30 patients with biopsy-proven hepatitis C virus-related liver disease participated in this prospective study. Degree of fibrosis and histologic activity were scored according to the METAVIR classification. The percentage of involved hepatocytes was used to grade steatosis. Hepatic spectra were obtained with a 3-T spectroscopic system. Tenfold cross-validated stepwise discriminant analysis was performed to classify disease severity on the basis of the spectroscopic findings. RESULTS. There was a strong correlation between 1H-MR spectroscopically measured lipid concentration and the degree of steatosis at histologic examination (r = 0.9236, p <0.0001). This finding enabled clear separation of groups according to degree of histologically determined steatosis. Variation in lipid concentration was consistent with the degree of steatosis (r = 0.7265, p <0.0001) and stage of fibrosis (r = 0.8156, p <0.0001). In univariate analysis, concentrations of both choline-containing compounds and glutamine-glutamate complex had a direct correlation with histologic grade (p <0.0001) and degree of steatosis (p <0.0001) but not with stage of fibrosis (p > 0.05). In multivariate analysis, the only factor independently associated with concentrations of choline-containing compounds and glutamine-glutamate complex was histologic grade. In cross-validated discriminant analysis based on choline-containing compound, glutamine-glutamate complex, and lipid resonance, 70% (21 of 30) of the histologic grade groups and 73% (22 of 30) of the steatosis groups were correctly classified. CONCLUSION. Hydrogen-1 MR spectroscopy can be an alternative to liver biopsy in the evaluation of steatosis and necroinflammatory activity in liver disease but is not useful for complete evaluation of hepatic fibrosis.
KW - H-MR spectroscopy
KW - Chronic hepatitis
KW - Liver fibrosis
KW - Liver steatosis
UR - http://www.scopus.com/inward/record.url?scp=43649105131&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=43649105131&partnerID=8YFLogxK
U2 - 10.2214/AJR.07.2262
DO - 10.2214/AJR.07.2262
M3 - Article
C2 - 18430852
AN - SCOPUS:43649105131
VL - 190
SP - 1331
EP - 1339
JO - American Journal of Roentgenology
JF - American Journal of Roentgenology
SN - 0361-803X
IS - 5
ER -