TY - JOUR
T1 - Evaluation of urinary biomarkers of exposure to benzene
T2 - Correlation with blood benzene and influence of confounding factors
AU - Hoet, Perrine
AU - Lison, Dominique
AU - Haufroid, Vincent
AU - De Smedt, Erika
AU - De Wilde, Peter
AU - Ferrari, Massimo
AU - Imbriani, Marcello
AU - Maestri, Luciano
AU - Negri, Sara
PY - 2009
Y1 - 2009
N2 - Purpose: trans,trans-Muconic acid (t,t- MA) is generally considered as a useful biomarker of exposure to benzene. However, because of its lack of specificity, concerns about its value at low level of exposure have recently been raised. The aim of this study was (a) to compare t,t-MA, S-phenylmercapturic acid (SPMA) and benzene (B-U) as urinary biomarkers of exposure to low levels of benzene in petrochemical workers and, (b) to evaluate the influence of sorbic acid (SA) and genetic polymorphisms of biotransformation enzymes on the excretion of these biomarkers. Method: A total of 110 workers (including 24 smokers; 2-10 cigarettes/ day) accepted to take part in the study. To assess external exposure to benzene, air samples were collected during the whole working period by a passive sampling device attached close to the breathing zone of 98 workers. Benzene was measured in blood (B-B) samples taken at the end of the shift, and was considered as the reference marker of internal dose. Urine was collected at the end of the shift for the determination of B-U, SPMA, t,t-MA, SA and creatinine (cr). B-U and B-B were determined by head-space/GC-MS, SPMA and SA by LC-MS, t,t-MA by HPLC-UV. Results: Most (89%) personal measurements of airborne benzene were below the limit of detection (0.1 ppm); B-B ranged from
AB - Purpose: trans,trans-Muconic acid (t,t- MA) is generally considered as a useful biomarker of exposure to benzene. However, because of its lack of specificity, concerns about its value at low level of exposure have recently been raised. The aim of this study was (a) to compare t,t-MA, S-phenylmercapturic acid (SPMA) and benzene (B-U) as urinary biomarkers of exposure to low levels of benzene in petrochemical workers and, (b) to evaluate the influence of sorbic acid (SA) and genetic polymorphisms of biotransformation enzymes on the excretion of these biomarkers. Method: A total of 110 workers (including 24 smokers; 2-10 cigarettes/ day) accepted to take part in the study. To assess external exposure to benzene, air samples were collected during the whole working period by a passive sampling device attached close to the breathing zone of 98 workers. Benzene was measured in blood (B-B) samples taken at the end of the shift, and was considered as the reference marker of internal dose. Urine was collected at the end of the shift for the determination of B-U, SPMA, t,t-MA, SA and creatinine (cr). B-U and B-B were determined by head-space/GC-MS, SPMA and SA by LC-MS, t,t-MA by HPLC-UV. Results: Most (89%) personal measurements of airborne benzene were below the limit of detection (0.1 ppm); B-B ranged from
KW - Benzene
KW - Biological monitoring
KW - S-Phenylmercapturic acid
KW - Sorbic acid
KW - t,t-Muconic acid
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U2 - 10.1007/s00420-008-0381-6
DO - 10.1007/s00420-008-0381-6
M3 - Article
C2 - 19009306
AN - SCOPUS:68449087193
VL - 82
SP - 985
EP - 995
JO - International Archives of Occupational and Environmental Health
JF - International Archives of Occupational and Environmental Health
SN - 0340-0131
IS - 8
ER -