Evidence for a Human Immunodeficiency Virus Type 1-Mediated Suppression of Uninfected Hematopoietic (CD34+) Cells in AIDS Patients

Giorgio Zauli, Maria Carla Re, Giuseppe Visani, Giuliano Furlini, Patrizio Mazza, Monica Vignoli, Michele LaPlaca

Research output: Contribution to journalArticle

Abstract

Hematopoietic progenitor (CD34+) cells were purified from the bone marrow of 6 human immunodeficiency virus (HIV) type 1-seropositive cytopenic patients and 10 healthy donors. HIV-1-seropositive patients showed a reduced number of granulocyte/macrophage, erythroid, and megakaryocyte progenitors and also a progressive and significant decline of numbers of CD34+ cells in liquid culture, which did not result from a productive or latent HIV-1 infection of CD34+cells. However, all HIV-1-seropositive patients showed signs of active viral replication at the bone marrow level. Moreover, virus isolates from 3 HIV-l-seropositive patients showed a dose-dependent inhibition on growth of normal CD34+cells. This suppressive activity was almost completely reversed by incubating the virus isolates with an anti-gp120 polyclonal antibody before adding to normal CD34+cells.

Original languageEnglish
Pages (from-to)710-716
Number of pages7
JournalJournal of Infectious Diseases
Volume166
Issue number4
DOIs
Publication statusPublished - 1992

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ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy
  • Immunology
  • Public Health, Environmental and Occupational Health

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