Evidence for a pathogenic role of extrafollicular, IL-10-producing CCR6 + B helper T cells in systemic lupus erythematosus

F. Facciotti, P. Larghi, R. Bosotti, C. Vasco, N. Gagliani, C. Cordiglieri, S. Mazzara, V. Ranzani, E. Rottoli, S. Curti, A. Penatti, B. Karnani, Y. Kobayashi, M. Crosti, M. Bombaci, J. P. Van Hamburg, G. Rossetti, R. Gualtierotti, M. Gerosa, S. GattiS. Torretta, L. Pignataro, S. W. Tas, S. Abrignani, M. Pagani, F. Grassi, P. L. Meroni, R. A. Flavell, J. Geginat

Research output: Contribution to journalArticle

Abstract

Interleukin 10 (IL-10) is an antiinflammatory cytokine, but also promotes B cell responses and plays a pathogenic role in systemic lupus erythematosus (SLE). CD4+CCR6+IL-7R+T cells from human tonsils produced IL-10 following stimulation by naïve B cells, which promoted B cell immunoglobulin G (IgG) production. These tonsillar CCR6+B helper T cells were phenotypically distinct from follicular helper T (TFH) cells and lacked BCL6 expression. In peripheral blood, a CCR6 + T cell population with similar characteristics was identified, which lacked Th17- and TFH-associated gene signatures and differentiation-associated surface markers. CD4+CCR6+T cells expressing IL-10, but not IL-17, were also detectable in the spleens of cytokine reporter mice. They provided help for IgG production in vivo, and expanded systemically in pristane-induced lupus-like disease. In SLE patients, CD4+CCR6+IL-7R+T cells were associated with the presence of pathogenic anti-dsDNA (doublestranded DNA) antibodies, and provided spontaneous help for autoantibody production ex vivo. Strikingly, IL-10-producing CCR6+T cells were highly abundant in lymph nodes of SLE patients, and colocalized with B cells at the margins of follicles. In conclusion, we identified a previously uncharacterized population of extrafollicular B helper T cells, which produced IL-10 and could play a prominent pathogenic role in SLE.

Original languageEnglish
Pages (from-to)7305-7316
Number of pages12
JournalProceedings of the National Academy of Sciences of the United States of America
Volume117
Issue number13
DOIs
Publication statusPublished - Mar 31 2020

Keywords

  • B cell help
  • Interleukin-10
  • Lupus

ASJC Scopus subject areas

  • General

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    Facciotti, F., Larghi, P., Bosotti, R., Vasco, C., Gagliani, N., Cordiglieri, C., Mazzara, S., Ranzani, V., Rottoli, E., Curti, S., Penatti, A., Karnani, B., Kobayashi, Y., Crosti, M., Bombaci, M., Van Hamburg, J. P., Rossetti, G., Gualtierotti, R., Gerosa, M., ... Geginat, J. (2020). Evidence for a pathogenic role of extrafollicular, IL-10-producing CCR6 + B helper T cells in systemic lupus erythematosus. Proceedings of the National Academy of Sciences of the United States of America, 117(13), 7305-7316. https://doi.org/10.1073/pnas.1917834117