Evidence for a role of platelet activating factor in hypovolemic shock in the rat

B. Zingarelli, F. Squadrito, F. Bussolino, G. Calapai, D. Altavilla, M. Ioculano, G. M. Campo, P. Canale, A. P. Caputi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

An extremely severe circulatory shock was produced in two different manners in urethane-anaesthetized rats in order to evaluate the key role of platelet-activating factor (PAF) and myocardial depressant factor (MDF) in low flow states. Haemorrhagic shock was induced by intermittently withdrawing about 50% of the estimated blood volume until mean arterial blood pressure (MAP) stabilized in the range of 20-25 mmHg. Vehicle-treated shocked rats died within 20-30 min after the last bleeding and exhibited elevated plasma activity of MDP (159.6 ± 7.4 U/ml). Treatment with a specific PAF receptor antagonist, L-659,989, at doses of 500 or 1000 nmol/kg i.v., significantly increased survival rate, blunted the rise in plasma MDF activity and maintained MAP at higher values compared to vehicle shocked rats. Similarly, in another group of rats PAF (15 nmol/kg, i.v.) produced a shock-like state characterized by a serious hypotension in the range of 20-30 mmHg, elevated plasma MDF activity (79.7 ± 7,7 U/ml) and death within 20-25 min after administration. L-659,989, given 5 min after the PAF-induced sharp decrease of MAP, improved survival rate, ameliorated MAP and reduced plasma levels of MDF. The results of this study, therefore, confirm that PAF plays a role in cardiovascular changes of hypovolemic circulatory shock both directly and by inducing the release of other factors such as MDF.

Original languageEnglish
Pages (from-to)123-134
Number of pages12
JournalJournal of Lipid Mediators and Cell Signalling
Volume9
Issue number2
Publication statusPublished - 1994

Fingerprint

Platelet Activating Factor
Myocardial Depressant Factor
Shock
Arterial Pressure
L 659989
Hemorrhagic Shock
Urethane
Blood Volume
Hypotension
Hemorrhage

Keywords

  • hypovolemic haemorrhagic shock
  • myocardial depressant factor
  • platelet-activating factor

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

Cite this

Zingarelli, B., Squadrito, F., Bussolino, F., Calapai, G., Altavilla, D., Ioculano, M., ... Caputi, A. P. (1994). Evidence for a role of platelet activating factor in hypovolemic shock in the rat. Journal of Lipid Mediators and Cell Signalling, 9(2), 123-134.

Evidence for a role of platelet activating factor in hypovolemic shock in the rat. / Zingarelli, B.; Squadrito, F.; Bussolino, F.; Calapai, G.; Altavilla, D.; Ioculano, M.; Campo, G. M.; Canale, P.; Caputi, A. P.

In: Journal of Lipid Mediators and Cell Signalling, Vol. 9, No. 2, 1994, p. 123-134.

Research output: Contribution to journalArticle

Zingarelli, B, Squadrito, F, Bussolino, F, Calapai, G, Altavilla, D, Ioculano, M, Campo, GM, Canale, P & Caputi, AP 1994, 'Evidence for a role of platelet activating factor in hypovolemic shock in the rat', Journal of Lipid Mediators and Cell Signalling, vol. 9, no. 2, pp. 123-134.
Zingarelli, B. ; Squadrito, F. ; Bussolino, F. ; Calapai, G. ; Altavilla, D. ; Ioculano, M. ; Campo, G. M. ; Canale, P. ; Caputi, A. P. / Evidence for a role of platelet activating factor in hypovolemic shock in the rat. In: Journal of Lipid Mediators and Cell Signalling. 1994 ; Vol. 9, No. 2. pp. 123-134.
@article{5b0567099d9d41fa8219febd860f8965,
title = "Evidence for a role of platelet activating factor in hypovolemic shock in the rat",
abstract = "An extremely severe circulatory shock was produced in two different manners in urethane-anaesthetized rats in order to evaluate the key role of platelet-activating factor (PAF) and myocardial depressant factor (MDF) in low flow states. Haemorrhagic shock was induced by intermittently withdrawing about 50{\%} of the estimated blood volume until mean arterial blood pressure (MAP) stabilized in the range of 20-25 mmHg. Vehicle-treated shocked rats died within 20-30 min after the last bleeding and exhibited elevated plasma activity of MDP (159.6 ± 7.4 U/ml). Treatment with a specific PAF receptor antagonist, L-659,989, at doses of 500 or 1000 nmol/kg i.v., significantly increased survival rate, blunted the rise in plasma MDF activity and maintained MAP at higher values compared to vehicle shocked rats. Similarly, in another group of rats PAF (15 nmol/kg, i.v.) produced a shock-like state characterized by a serious hypotension in the range of 20-30 mmHg, elevated plasma MDF activity (79.7 ± 7,7 U/ml) and death within 20-25 min after administration. L-659,989, given 5 min after the PAF-induced sharp decrease of MAP, improved survival rate, ameliorated MAP and reduced plasma levels of MDF. The results of this study, therefore, confirm that PAF plays a role in cardiovascular changes of hypovolemic circulatory shock both directly and by inducing the release of other factors such as MDF.",
keywords = "hypovolemic haemorrhagic shock, myocardial depressant factor, platelet-activating factor",
author = "B. Zingarelli and F. Squadrito and F. Bussolino and G. Calapai and D. Altavilla and M. Ioculano and Campo, {G. M.} and P. Canale and Caputi, {A. P.}",
year = "1994",
language = "English",
volume = "9",
pages = "123--134",
journal = "Journal of Lipid Mediators and Cell Signalling",
issn = "0929-7855",
publisher = "Elsevier BV",
number = "2",

}

TY - JOUR

T1 - Evidence for a role of platelet activating factor in hypovolemic shock in the rat

AU - Zingarelli, B.

AU - Squadrito, F.

AU - Bussolino, F.

AU - Calapai, G.

AU - Altavilla, D.

AU - Ioculano, M.

AU - Campo, G. M.

AU - Canale, P.

AU - Caputi, A. P.

PY - 1994

Y1 - 1994

N2 - An extremely severe circulatory shock was produced in two different manners in urethane-anaesthetized rats in order to evaluate the key role of platelet-activating factor (PAF) and myocardial depressant factor (MDF) in low flow states. Haemorrhagic shock was induced by intermittently withdrawing about 50% of the estimated blood volume until mean arterial blood pressure (MAP) stabilized in the range of 20-25 mmHg. Vehicle-treated shocked rats died within 20-30 min after the last bleeding and exhibited elevated plasma activity of MDP (159.6 ± 7.4 U/ml). Treatment with a specific PAF receptor antagonist, L-659,989, at doses of 500 or 1000 nmol/kg i.v., significantly increased survival rate, blunted the rise in plasma MDF activity and maintained MAP at higher values compared to vehicle shocked rats. Similarly, in another group of rats PAF (15 nmol/kg, i.v.) produced a shock-like state characterized by a serious hypotension in the range of 20-30 mmHg, elevated plasma MDF activity (79.7 ± 7,7 U/ml) and death within 20-25 min after administration. L-659,989, given 5 min after the PAF-induced sharp decrease of MAP, improved survival rate, ameliorated MAP and reduced plasma levels of MDF. The results of this study, therefore, confirm that PAF plays a role in cardiovascular changes of hypovolemic circulatory shock both directly and by inducing the release of other factors such as MDF.

AB - An extremely severe circulatory shock was produced in two different manners in urethane-anaesthetized rats in order to evaluate the key role of platelet-activating factor (PAF) and myocardial depressant factor (MDF) in low flow states. Haemorrhagic shock was induced by intermittently withdrawing about 50% of the estimated blood volume until mean arterial blood pressure (MAP) stabilized in the range of 20-25 mmHg. Vehicle-treated shocked rats died within 20-30 min after the last bleeding and exhibited elevated plasma activity of MDP (159.6 ± 7.4 U/ml). Treatment with a specific PAF receptor antagonist, L-659,989, at doses of 500 or 1000 nmol/kg i.v., significantly increased survival rate, blunted the rise in plasma MDF activity and maintained MAP at higher values compared to vehicle shocked rats. Similarly, in another group of rats PAF (15 nmol/kg, i.v.) produced a shock-like state characterized by a serious hypotension in the range of 20-30 mmHg, elevated plasma MDF activity (79.7 ± 7,7 U/ml) and death within 20-25 min after administration. L-659,989, given 5 min after the PAF-induced sharp decrease of MAP, improved survival rate, ameliorated MAP and reduced plasma levels of MDF. The results of this study, therefore, confirm that PAF plays a role in cardiovascular changes of hypovolemic circulatory shock both directly and by inducing the release of other factors such as MDF.

KW - hypovolemic haemorrhagic shock

KW - myocardial depressant factor

KW - platelet-activating factor

UR - http://www.scopus.com/inward/record.url?scp=0028282054&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028282054&partnerID=8YFLogxK

M3 - Article

VL - 9

SP - 123

EP - 134

JO - Journal of Lipid Mediators and Cell Signalling

JF - Journal of Lipid Mediators and Cell Signalling

SN - 0929-7855

IS - 2

ER -