Evidence for a specific role of vasopressin in sustaining pituitary-adrenocortical stress response in the rat

Sergio Scaccianoce, Luisa A A Muscolo, Giovanni Cigliana, Domitilla Navarra, Raffaella Nicolai, Luciano Angelucci

Research output: Contribution to journalArticlepeer-review

Abstract

In the adult male Wistar rat a 2-fold 2-min restraint stress exposure, repeated 15 min apart, activated the adrenocortical secretion more than a single one would have. However, in rats with a pharmacological block of the endogenous CRF release, exogenous CRH (0.3 μg/kg iv), administered 15 min after a first similar dose, was unable to stimulate pituitary-adrenocortical activity above the level attained with the first peptide injection. On the contrary, in the same conditions exogenous arginine vasopressin (AVP) (0.3 μg/kg iv) administered 15 min after CRH, was able to further stimulate pituitary-adrenocortical activity. Using the same experimental procedure, oxytocin (0.3 μg/kg iv) was found to be totally inactive. The physiological import of these findings was investigated in the Brattleboro rat, genetically lacking in endogenous AVP, in which, unlike the control Long-Evans strain, the 2-fold stress exposure did not cause an increase in plasma corticosterone concentration greater than that of a single exposure. These results suggest that endogenous AVP is essential in sustaining adrenocortical activation in circumstances in which pituitary refractoriness towards CRH stimulation intervenes.

Original languageEnglish
Pages (from-to)3138-3143
Number of pages6
JournalEndocrinology
Volume128
Issue number6
Publication statusPublished - Jun 1991

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

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