Evidence for cell surface association between CXCR4 and ganglioside GM3 after gp120 binding in SupT1 lymphoblastoid cells

Maurizio Sorice, Tina Garofalo, Roberta Misasi, Agostina Longo, Vincenzo Mattei, Patrizio Sale, Vincenza Dolo, Roberto Gradini, Antonio Pavan

Research output: Contribution to journalArticlepeer-review

Abstract

CXCR4 (fusin) is a chemokine receptor which is involved as a coreceptor in gp120 binding to the cell surface. In this study we provide evidence that binding of gp120 triggers CXCR4 recruitment to glycosphingolipid-enriched microdomains. Scanning confocal microscopy showed a nearly complete localization of CXCR4 within GM3-enriched plasma membrane domains of SupT1 cells and coimmunoprecipitation experiments revealed that CXCR4 was immunoprecipitated by IgG anti-GM3 after gp120 pretreatment. These findings reveal that gp120 binding induces a strict association between CXCR4 and ganglioside GM3, supporting the view that GM3 and CXCR4 are components of a functional multimolecular complex critical for HIV-1 entry.

Original languageEnglish
Pages (from-to)55-60
Number of pages6
JournalFEBS Letters
Volume506
Issue number1
DOIs
Publication statusPublished - Sep 28 2001

Keywords

  • CXCR4
  • Ganglioside
  • GM3
  • gp120
  • Microdomain

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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