Abstract
CXCR4 (fusin) is a chemokine receptor which is involved as a coreceptor in gp120 binding to the cell surface. In this study we provide evidence that binding of gp120 triggers CXCR4 recruitment to glycosphingolipid-enriched microdomains. Scanning confocal microscopy showed a nearly complete localization of CXCR4 within GM3-enriched plasma membrane domains of SupT1 cells and coimmunoprecipitation experiments revealed that CXCR4 was immunoprecipitated by IgG anti-GM3 after gp120 pretreatment. These findings reveal that gp120 binding induces a strict association between CXCR4 and ganglioside GM3, supporting the view that GM3 and CXCR4 are components of a functional multimolecular complex critical for HIV-1 entry.
Original language | English |
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Pages (from-to) | 55-60 |
Number of pages | 6 |
Journal | FEBS Letters |
Volume | 506 |
Issue number | 1 |
DOIs | |
Publication status | Published - Sep 28 2001 |
Keywords
- CXCR4
- Ganglioside
- GM3
- gp120
- Microdomain
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology