TY - JOUR
T1 - Evidence for covalent binding of adriamycin to rat liver microsomal proteins
AU - Ghezzi, Pietro
AU - Donelli, Maria Grazia
AU - Pantarotto, Claudio
AU - Facchinetti, Terenzio
AU - Garattini, Silvio
PY - 1981/1/15
Y1 - 1981/1/15
N2 - Adriamycin (NSC-123, 127) is an anticancer drug of the anthracycline type widely used in the clinical practice for the treatment of different malignancies (1). Intercalation with DNA has been suggested as having a role in the mechanism of action of this drug (2, 3, 4). Recently, reports by Sinha (5) and Lucacchini et al. (6) suggested a covalent interaction of Adriamycin (AM) with DNA and proteins. These studies, however, were not conclusive since they were carried out by spectrophotometric and Chromatographie procedures. In the present study a covalent interaction between AM and microsomal proteins was demonstrated in a more "classical" covalent binding study with the 14C-labelled compound.
AB - Adriamycin (NSC-123, 127) is an anticancer drug of the anthracycline type widely used in the clinical practice for the treatment of different malignancies (1). Intercalation with DNA has been suggested as having a role in the mechanism of action of this drug (2, 3, 4). Recently, reports by Sinha (5) and Lucacchini et al. (6) suggested a covalent interaction of Adriamycin (AM) with DNA and proteins. These studies, however, were not conclusive since they were carried out by spectrophotometric and Chromatographie procedures. In the present study a covalent interaction between AM and microsomal proteins was demonstrated in a more "classical" covalent binding study with the 14C-labelled compound.
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U2 - 10.1016/0006-2952(81)90191-X
DO - 10.1016/0006-2952(81)90191-X
M3 - Article
C2 - 7236308
AN - SCOPUS:0019364997
VL - 30
SP - 175
EP - 177
JO - Biochemical Pharmacology
JF - Biochemical Pharmacology
SN - 0006-2952
IS - 2
ER -