Evidence for GroES acting as a transcriptional regulator

Giuseppe Legname, Pasqualina Buono, Gianluca Fossati, Nicoletta Monzini, Paolo Mascagni, Daniela Modena, Fabrizio Marcucci

Research output: Contribution to journalArticle

Abstract

Cochaperonins (cpn10) assist chaperonins (cpn60) in promoting folding and assembly of other proteins. Upon expression of Mycobacterium tuberculosis cpn10 in Escherichia coli we have purified a polypeptide which, through amino acid sequencing, was identified as the endogenous E. coli 10K-S protein. Subsequent studies showed that its expression was specifically upregulated upon cloning of different members of the cpn10 family, including GroES, the E. coli cpn10. Pulse-chase experiments demonstrated that 10K-S is but one of several proteins whose expression is modulated upon cloning of cpn10. Up-regulation of 10K-S was also observed after exposure of normal cells, but not of groES- mutants, to elevated temperatures (42°C). This allowed us to define 10K-S as a heat-shock protein (hsp) whose expression is dependent on the production of another hsp, GroES. Northern blot experiments showed that enhanced expression of 10K-S was consequent to increased accumulation of transcripts and that groES- mutants were devoid even of baseline levels of transcripts both at 37°C and after temperature upshift. These results show that GroES, in addition to its established role in assisting protein folding may act as a transcriptional regulator and is likely to play an important role in modulating gene expression particularly in those conditions, like the stress response, in which its production is greatly enhanced.

Original languageEnglish
Pages (from-to)412-418
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume229
Issue number2
DOIs
Publication statusPublished - Dec 13 1996

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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    Legname, G., Buono, P., Fossati, G., Monzini, N., Mascagni, P., Modena, D., & Marcucci, F. (1996). Evidence for GroES acting as a transcriptional regulator. Biochemical and Biophysical Research Communications, 229(2), 412-418. https://doi.org/10.1006/bbrc.1996.1818