TY - JOUR
T1 - Evidence for preferred MHC class II-TCR recognition independent of the source of bound peptide
AU - Battaglia, Manuela
AU - Gorski, Jack
PY - 2002/8
Y1 - 2002/8
N2 - The interaction between TCR and peptide-MHC is well described in terms of the recognition of the peptide, but the recognition of the MHC is less well understood. At issue is whether particular V gene products may have higher affinity for some MHC over others and to what extent the bound peptide influences V gene selection. We examined this issue by developing T cell lines in which the presenting MHC class II molecule has a constant TCR contact region, while the presented peptides vary. If there is an affinity between particular V genes and the specific MHC used, only a subset of the V genes will be associated with the response. Indeed, in all the cell lines analyzed, there was a reproducible usage of a limited number of Vβ genes, regardless of the bound peptides. This Vβ-gene constraint was independent of the CDR3 sequence, compatible with the lack of involvement of specific peptides. Our results support the hypothesis that certain V gene products may have a preference for interacting with a particular MHC molecule, and this could have an impact in selectively controlling immune responses.
AB - The interaction between TCR and peptide-MHC is well described in terms of the recognition of the peptide, but the recognition of the MHC is less well understood. At issue is whether particular V gene products may have higher affinity for some MHC over others and to what extent the bound peptide influences V gene selection. We examined this issue by developing T cell lines in which the presenting MHC class II molecule has a constant TCR contact region, while the presented peptides vary. If there is an affinity between particular V genes and the specific MHC used, only a subset of the V genes will be associated with the response. Indeed, in all the cell lines analyzed, there was a reproducible usage of a limited number of Vβ genes, regardless of the bound peptides. This Vβ-gene constraint was independent of the CDR3 sequence, compatible with the lack of involvement of specific peptides. Our results support the hypothesis that certain V gene products may have a preference for interacting with a particular MHC molecule, and this could have an impact in selectively controlling immune responses.
KW - MHC
KW - T cell receptor
KW - T lymphocyte
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U2 - 10.1002/1521-4141(200208)32:8<2179::AID-IMMU2179>3.0.CO;2-P
DO - 10.1002/1521-4141(200208)32:8<2179::AID-IMMU2179>3.0.CO;2-P
M3 - Article
C2 - 12209630
AN - SCOPUS:0036687110
VL - 32
SP - 2179
EP - 2187
JO - European Journal of Immunology
JF - European Journal of Immunology
SN - 0014-2980
IS - 8
ER -